异烟肼对大鼠成骨细胞影响的观察  

Effect of isoniazid on the proliferation,alkaline phosphatase activity and type Ⅰ collagen synthesis of osteoblasts in rats

作　　者：刘江涛[1] 王永清[1] 张卫国[1] 葛保健[1] 叶锋 梁新军[1] 夏仁云[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院骨科,武汉市430030 [2]武汉市十一医院骨科,430015

出　　处：《中国脊柱脊髓杂志》2006年第12期924-926,共3页Chinese Journal of Spine and Spinal Cord

基　　金：内蒙古科技厅科技合作项目(2004006)

摘　　要：目的:研究异烟肼(isoniazid,INH)对大鼠成骨细胞(osteoblasts,OB)增殖、碱性磷酸酶(Alkalinephosphatase,ALP)活性和Ⅰ型胶原合成的影响。方法:分离、培养新生SD大鼠头盖骨OB,分别加入不同浓度的INH(1、10、20、40、60、100、1000滋g/ml)共同培养96h,对照组不加INH,分别采用四唑盐(MTT)比色实验、ALP活性测定法和3H-Proline(3氢-脯氨酸)法测定OB增殖、ALP活性和Ⅰ型胶原合成的情况。结果:与对照组比较,INH在60滋g/ml即可抑制大鼠OB增殖(P<0.05),40滋g/ml时大鼠成骨细胞ALP活性明显下降(P<0.05),20滋g/ml时大鼠成骨细胞Ⅰ型胶原合成(P<0.05)明显减少,且都随浓度增加抑制作用加强。结论:INH在较低浓度时对OB增殖、ALP活性和Ⅰ型胶原合成即有抑制作用,故在骨结核治疗中应该严格控制药物剂量,特别是在局部用药时。To investigate the effect of isoniazid （INH） on the proliferation,alkaline phosphatase （ALP） activity and type Ⅰ collagen synthesis of osteoblasts（OB） derived from newly born SD rat calvarias in vitro.Metbod：Osteoblasts derived from newly born （〈24h） SD rats＇ calvarias were cultured with different concentration （1,10,20,40,60,100, 1000μg/ml） of INH.After 96h,MTT,ALP activity and ^3H-Proline incorporation were measured respectively.Result：96h after adding INH,the MTT A490 values of OB were significantly decreased at the concentration of 60μg/ml comparing to that of control group （P〈0.05）,and those were much lower in the presence of INH（100,1000μg/ml）（P〈0.01 vs. control group）.The ALP activity of OB was significantly decreased at the concentration of 40μg/ml comparing to that of control group （P〈0.05）,and it was much lower in the presence of INH （60,100,1000μg/ml） （P〈0.01 vs. control group）.In the type Ⅰ collagen synthesis,3H-Proline incorporation of OB was significantly decreased at the concentration of 20μg/ml comparing to that of control group （P〈0.05）,and it was much lower in the presence of INH（40,60,100, 1000μg/ml） （P〈0.01 vs. control group ）.Conclusion ： It has shown that INH at the lower concentration can inhibit the proliferation,ALP activity and synthesis of type Ⅰ collagen of OB in vitro.Further studies should be carried out to assess whether INH is detrimental to the bone repair process in vivo.

关 键 词：异烟肼 成骨细胞 碱性磷酸酶 Ⅰ型胶原 大鼠 

分 类 号：R978.3[医药卫生—药品] R529.2[医药卫生—药学]