Mitochondrial DNA D-loop Variation and Genetic Background of Brahman Cattle  

婆罗门牛mtDNA D-loop变异及其遗传背景(英文)

作　　者：亐开兴[1,2] 吴桂生[2] 廖祥龙[1] 金显栋[1] 赵刚[1] 杨国荣[1] 袁希平[1] 黄必志[1] 文际坤[1] 张亚平[2] 

机构地区：[1]云南省肉牛和牧草研究中心,云南昆明650212 [2]中国科学院昆明动物研究所云南省畜禽分子生物学重点实验室,分子进化与基因组多样性实验室

出　　处：《Zoological Research》2006年第6期615-620,共6页动物学研究（英文）

基　　金：云南省自然科学基金(2003C0011Z,2004YX41);云南省科技厅科技项目(2004NG04)

摘　　要：The complete mitochondrial DNA D-loop sequences from 10 stud Brahman cattle were sequenced and analyzed. The results showed that the genetic diversity of Brahman cattle was rich ; the rate of nucleotide variation, haplotype diversity and nucleotide diversity were 6.25%, 0.978± 0.054 and 0.014 30± 0.008 68, respectively. Nine haplotypes were defined and fell into two distinct lineages, suggesting that Brahman cattle have both Bos indicus （Zebu） and B. taurus genetic background. The taurine haplotypes were predominant at 90% and only Brah-6 belonged to the Asian zebu mthaplotype. This indicates that Brahman cattle was one of the zebu breeds and inherited the excellent characteristics of both the Asian zebu and European beef cattle, such as easy calf delivery, high quality beef, heat tolerance and resistance to various parasites. Breeders introduced Brahman cattle to improve the productivity and adaptability of native cattle. The Zebu has evidently frequently introgressed into the modem taurine breeds. As for modem zebu breeds, B. taurus also highly contributed to their formation, except for the Asian zebu. Furthermore our results also confirm the hypothesis that B. indicus has undergone a separate domestication event and originated from the Indian subcontinent.对10头原种婆罗门牛mtDNAD-loop全序列912 bp测序,婆罗门牛遗传多样性丰富,检测到的9种单倍型兼有瘤牛(B.indicus)与普通牛(B.taurus)的遗传背景,核苷酸变异率为6.25 %,单倍型多态度为0.978±0.054 ,核苷酸多态度为0.014 30±0.008 68。所有单倍型聚为明显的两大分支,婆罗门牛的大部分单倍型为普通牛单倍型类群,并占绝对优势(90 %) ,仅Brah-6与亚洲瘤牛聚在一起,属于亚洲瘤牛线粒体单倍型,表明婆罗门牛的确是集亚洲瘤牛、欧洲普通牛等优良特性于一身(易产犊、产肉性能好、耐热与体表寄生虫等)的瘤牛品种之一。育种学家引种瘤牛的目的是改善当地牛的生产力与适应性,现代普通牛表现出明显又普遍的瘤牛渐渗现象。对现代的瘤牛品种而言,除亚洲瘤牛品种外,普通牛对其他瘤牛品种育成的贡献同样高。支持瘤牛(B.indicus)为独立驯化、起源于印度次大陆的假说。

关 键 词：Brahman cattle mtDNA D-loop polymorphism genetic background 

分 类 号：Q953[生物学—动物学]