DRE-PCR分型方法在唐山市结核分枝杆菌研究中的应用  

Application of the double repetitive element-PCR (DRE-PCR) based DNA fingerprinting analysis to the genotyping of Mycobacterium tuberculosis

作　　者：王东[1] 冯福民[1] 刘茜[1] 郭梅[1] 曹燕花[1] 李世明[2] 么作义 高宝霞[1] 孙永红[1] 

机构地区：[1]华北煤炭医学院预防医学系流行病与卫生统计学科,唐山063000 [2]唐山市结核病医院

出　　处：《中国人兽共患病学报》2006年第12期1143-1146,共4页Chinese Journal of Zoonoses

基　　金：卫生部科技攻关项目子课题;No.2003BA712A11-24

摘　　要：目的DRE-PCR DNA指纹分型方法的建立及其在分枝杆菌基因分型中的应用。方法收集结核病患者痰标本，分离培养获得分枝杆菌，进行表型鉴定及耐药性检测；提取基因组DNA，在建立DRE-PCR DNA指纹分型方法的基础上对分枝杆菌进行DNA指纹图谱分析。结果DRE-PCR指纹图谱分析将80株分枝杆菌分为13种菌型，其中A型菌26株（32．5％），B型菌14株（17．5％），C型菌16株（20．0％），其余各型均〈7．5％。分组统计显示，3种主要的DRE-PCR DNA指纹在40～60岁组的分布偏高（P=0．019），而与患者的性别、职业、长期居住地、结核接触史、痰涂片、耐药性等无关。结论DRE-PCR DNA指纹分型方法是一种简单、快速的分枝杆菌的分型方法；3种主要类型的结核分枝杆菌在唐山地区流行。To establish the double repetitive element-PCR （DRE-PCR） molecular typing method to be used in the genotyping of Mycobacterium tuberculosis （MTB）, 80 sputum specimens of patients with pulmonary tuberculosis were collected and cultivated at 37℃ to obtain the clinical isolates of MTB. These isolates were identified by means of the phenotyple method. And their chromosomal DNA was extracted. The DNA-fingerprinting analysis of MTB was performed on the basis of the established method of DRE-PCR. As analyzed with this method, the 80 MTB isolates were finally classified into 13 genotypes, in which most of them belonged to A （32.5%）, B （17.5%） and C （20.0%） genotypes and the other 10 genotypes appeared to be scattered. There was a significant difference among different age groups in the DRE-PCR fingerprinting. This result indicates that the DRE-PCR based DNA fingerprinting analysis is proved to be a fast and simple molecular typing method used for the genotyping, and there exists three main genotypes in the epidemic strains of Mycobacterium tuberculosis, in Tangshan city.

关 键 词：DRE-PCR结核分枝杆菌 基因分型 

分 类 号：R378[医药卫生—病原生物学]