结核分枝杆菌cfp10-esat6融合基因重组穿梭质粒的构建及其在BCG中的融合与分泌表达研究  

Construction of the Recombinant Integrating Shuttle Plasmid with cfp10-esat6 Fusion Gene of Mycobacterium Tuberculosis and Its Expression in BCG

作　　者：王晓樱[1] 鲍朗[1] 赵明才[1] 张会东[1] 龙洋[1] 

机构地区：[1]四川大学华西基础医学与法医学院感染免疫研究室,成都610041

出　　处：《生物医学工程学杂志》2006年第6期1298-1302,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30271172)

摘　　要：通过SOE法(重叠延伸,Genesplicingbyoverlapextension),扩增cfp10-esat6融合基因,与穿梭整合质粒pMV361构建成重组质粒。另将BCGα抗原(α-Ag)信号肽序列与上述重组质粒构建成另一重组质粒。将两种重组质粒分别导入BCG菌构建融合型重组卡介苗和分泌型重组卡介苗,热诱导后分析其表达产物。本研究成功构建并表达了CFP10-ESAT6融合及分泌表达的重组BCG,为发展新型结核病疫苗打下了一定的基础。This study was conducted to amplify the cfp10-esat6 fusion gene by SOE and insert into the integrating shuttle plasmid pMV361 to form the recombinant plasmid. Then another recombinant plasmid was constructed by insertingα-A g signal sequence of BCG. The two recombinant plasmids were introduced into BCG and the induced products from recombinant BCG were analyzed. In conclusion,the successful construction of rBCG expressing the fusion protein CFP10-ESAT6 will be the base of the development of novel Mycobacterium tuberclosis vaccines.

关 键 词：结核分枝杆菌 CFP10-ESAT6融合蛋白 整合穿梭质粒pMV36 卡介苗 

分 类 号：R346[医药卫生—基础医学]