红景天苷对骨髓抑制贫血小鼠骨髓基质金属蛋白酶的影响  

Effects of Salidroside on Bone Marrow Matrix Metalloproteinases of Bone Marrow Depressed Anemic Mice

作　　者：张新胜[1] 祝彼得[2] 金沈瑞[2] 严苏纯[2] 陈志伟[2] 

机构地区：[1]四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室,成都610041 [2]成都中医药大学,成都610075

出　　处：《生物医学工程学杂志》2006年第6期1314-1319,共6页Journal of Biomedical Engineering

摘　　要：本研究用免疫组织化学方法和酶谱电泳法观察了红景天苷对骨髓抑制贫血小鼠骨髓基质金属蛋白酶-2和-9(MMP-2、MMP-9)的影响,并探讨其在造血调控中的可能作用。免疫组化结果显示,各组小鼠骨髓细胞中均检测到MMP-2和MMP-9表达。与对照组相比,模型组及高、中、低剂量红景天苷组骨髓细胞中MMP-2、MMP-9蛋白表达明显增强。在造模后第4d,中剂量红景天苷组中MMP-2和MMP-9表达最强。在8d,分别是低剂量和中剂量红景天苷组中MMP-2和MMP-9表达最强。酶谱电泳结果显示,在对照组骨髓中可检测到66kD(MMP-2的酶原形式,proMMP-2)、62kD(MMP-2的活性形式,MMP-2)、86kD(MMP-9活性形式,MMP-9)和94kD(MMP-9的酶原形式,proMMP-9)4条明胶酶的活性带,其中MMP-9的活性最强。造模后骨髓造血微环境中明胶酶的活性明显降低,不同剂量的红景天苷均能明显促进proMMP-9和MMP-9的活性增强,使proMMP-2的活性减弱。结果表明,红景天苷可能通过促进骨髓细胞中基质金属蛋白酶表达增强、骨髓造血微环境中基质金属蛋白酶活性升高,进而导致ECM中或基质细胞膜上细胞因子的释放、骨髓微血管损伤的修复以及HSCs增殖、迁移和分化能力的增加来促进骨髓造血功能的恢复。To examine the effect of salidroside on the expression and activities of matrix metalloproteinase-2 （MMP-2） and matrix metalloproteinase-9 （MMP-9） in bone marrow （BM）of BM depressed anemic mice by immunohistoehemistry and gelatin zymography respectively, and to explore its roles in hematopoietie regulation. Immunohistoehemistry showed that the expression of MMP-2 and MMP-9 of bone marrow eells（BMCs） was found in each group. Compared with control group, the expression of MMP-2 and MMP-9 was obviously increased in the model group, low-dose, middle-dose and high-dose salidroside . At day 4 after treatment of radiation and chemotherapy, the peak of the expression of MMP-2 and MMP-9 was found in middle-dose salidroside . At day 8 after treatment of radiation and chemotherapy, the peak of the expression of MMP-2 and MMP-9 was found in low-dose and middle-dose salidroside respectively. Gelatin zymography revealed that 66 kD proMMP-2, 62 kD MMP-2, 86 kD MMP-9 and 94 kD proMMP-9 were detected in control group, and the activity of MMP-9 was stronger among them. After treatment of radiation and chemotherapy, the activity of gelatinases of hemopoietic mieroenviroment （HM） was obviously decreased , but low-dose, middle-dose and high-dose salidroside could significantly increase the activities of proMMP-9 and MMP-9, attenuate the activity of proMMP-2. These results suggest that salidroside could promote the recovery of hematopoietie function of BM depressed anemic mice by increasing the expression and activity of MMPs, releasing the eytokines from ECM or cell membrane, repairing impaired mierovessels of HM and promotion proliferation, migration and differentiation of HSCs.

关 键 词：红景天苷 骨髓抑制 骨髓细胞 基质金属蛋白酶-2 基质金属蛋白酶-9 

分 类 号：R285[医药卫生—中药学]