茶多酚对离体大鼠尾动脉血管环收缩性能的作用  

Effect of tea polyphenols on the contractility of isolated rat-tail artery ring

作　　者：曲直立[1] 李卫平[1] 王丽萍[2] 杨丽松[1] 周琴[1] 韩国柱[1] 

机构地区：[1]大连医科大学药理学教研室,辽宁省大连市116023 [2]大连大学附属医院心内科,辽宁省大连市116021

出　　处：《中国临床康复》2006年第47期71-74,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察来源于植物茶叶中的茶多酚对离体大鼠尾动脉血管在正常、氯化钾诱导、去甲肾上腺素诱导、无钙环境等状态下收缩性能的影响。方法:实验于2004-10/2005-03在大连医科大学中心实验室进行。①选取28只健康SD大鼠用于尾动脉血管环的制备。茶多酚静脉注射液(10g/L,临用前以生理盐水稀释)与溶媒均由大连理工大学李楠教授馈赠。重酒石酸去甲肾上腺素(上海禾丰制药有限公司产品,批号3E20003)。②28只大鼠乙醚麻醉,仰位固定,尾腹侧正中纵切,迅速取出一段动脉血管,制备动脉环,共制28个。动脉环一侧固定于含20mLK-H液的浴槽底部,另一侧与LW-10型医用张力传感器和BI-410生物机能实验系统相连。血管平滑肌收缩通过张力换能器以电压的形式记录于生物机能实验系统。降低率(%)=(1-给药后振幅平均值/给药前振幅平均值)×100%。③正常离体血管平滑肌收缩实验:将茶多酚静脉注射液以生理盐水稀释,分为0.5×10-6,1.0×10-6,2.0×10-6,4.0×10-6,8.0×10-6,1.6×10-5g/L浓度组,另设立溶媒对照组。本项实验选取6个动脉环,每个均用于不同茶多酚浓度及溶媒的检测,每做完一种浓度后,血管环用保养液清洗进行平衡,再做下一种浓度。动脉环制备及系统安装如上,血管平衡1h后,各茶多酚浓度组分别加入对应浓度的茶多酚0.2mL,溶媒对照组加入溶媒0.2mL。④氯化钾诱导离体血管收缩实验:将茶多酚静脉注射液以生理盐水稀释,分为2.0×10-6,4.0×10-6,8.0×10-6,1.6×10-5,3.2×10-5g/L浓度组,另设立溶媒对照组。本项实验选取8个动脉环,血管平衡1h后,加入氯化钾预收缩,5min后换液,重复1次,当收缩达到最高时,各茶多酚浓度组分别加入对应浓度的茶多酚0.2mL,溶媒对照组加入溶媒0.2mL。⑤去甲肾上腺素诱导离体血管收缩实验:分组情况与氯化钾诱导离体血管收缩实验相同,但本项实验选取7个动脉环,血管平AIM： To observe the effects of tea polyphenols （TP） on the contract function of isolated rat-tail artery under normal condition, induction of potassium chloride （KCI）, induction of noradrenaline （NA） and calcium-free condition. METHODS： The experiment was conducted in the Central Laboratory of Dalian Medical University from October 2004 to March 2005.① Twenty-eight healthy SD rats were selected for preparation of tail artery ring. Venous TP solution （10 g/L, diluted with normal saline before using） and menstruum were provided by Professor Li Nan of Dalian University of Technology. The levarterenol bitartrate was manufactured by Shanghai Hefeng Pharmaceutical Co., Ltd with the batch number of 3E20003.② Twenty-eight ruts were anestlletized with diethyl ether, fixed on back and longitudinally cut in ventral median tail to quickly obtain a segment of arterial vessel for preparation of 28 artery rings. Side of the artery ring was fixed at the bottom of bath bottle which containing 20 mL of K-H fluid, while the other side was connected with LW-10 medical tension transducer and BI-410 biofunction experiment system. The contraction of vascular smooth muscle was recorded into the biofunction experiment system by tension transducer. Decreasing rate （%） =（1-mean amplitude after administration/mean amplitude before administration）×100%. ③Experiment of contraction of isolated vascular smooth muscle under normal condition： TP solution was diluted by normal saline and divided into several groups according to different concentrations： 0.5×10^-6, 1.0×10^-6, 2.0×10^-6, 4.0×10^-6, 8.0×10^-6 and 1.6×10^-5 g/L, while administration of menstruum was considered as the control group. Six artery rings were adopted in the experiment for detection of dissolvent and TP of different concentrations. The artery ring was watered with nutrient fluid to keep equilibrium after detection, and it was then adopted in the next test. The preparation and system installation was the same as above, and 0.2 mL o

关 键 词：茶 酚类/药理学 去甲肾上腺素 钙通道/代谢 血管 大鼠 

分 类 号：R285[医药卫生—中药学]