江浙蝮蛇毒纤溶酶的生化和药理性质特征(英文)  

Biochemical and pharmacological characteristics of a fibrinolytic enzyme from Agkistrodon halys Pallas venom in Jiangzhe region

作　　者：张学荣[1] 张箴波[1] 舒雨雁[1] 蒙怡[1] 刘海涛[1] 

机构地区：[1]广西医科大学蛇毒研究所,广西壮族自治区南宁市530021

出　　处：《中国临床康复》2006年第47期180-182,188,共4页Chinese Journal of Clinical Rehabilitation

基　　金：广西壮族自治区科学技术厅资助项目(桂科基0342002-6);广西壮族自治区教育厅资助项目(桂教科研[2002]121)~~

摘　　要：背景:蝰亚科、蝮亚科和眼镜蛇科等蛇毒中存在着一类能直接溶解纤维蛋白(原)的酶,称为纤维蛋白(原)溶酶(纤溶酶),可做为溶解在心肌梗死、中风等期间形成的血栓的抗栓药物。目的:分离纯化江浙蝮蛇毒纤溶酶,并分析其部分生物化学和药理性质。设计:对照观察实验。单位:广西医科大学蛇毒研究所。材料:实验于2003-07/2005-06在广西医科大学蛇毒研究所完成。昆明种小鼠,体质量18～25g,平均(20±2)g,鼠龄三四个月,由广西医科大学实验动物中心提供。蛇毒冻干粉由广西医科大学蛇毒研究所提供。方法:应用DEAESepharoseCL-6B阴离子交换柱和HiPrepSephacrylS100柱分离纯化纤溶酶,以高效液相色谱仪和聚丙烯酰胺凝胶电泳检测纯度、相对分子质量,应用等电聚焦电泳测定等电点,然后测定氨基酸组分及部分药理性质。主要观察指标:纤溶酶相对分子质量、等电点和氨基酸组成,抑制剂对纤溶酶的作用,出血活性实验,纤溶酶对家兔体内抗凝活性的作用。结果:纳入30只鼠和12只兔全部进入结果分析。分离得到相对分子质量为59100,等电点为4.98,SDS-PAGE为一条带,高效液相色谱分析为单一峰的纤溶酶。该组分属于金属蛋白酶类。氨基酸组成分析表明它含较多的酸性氨基酸。有微弱的出血毒性。具有较强且持久的溶栓效果。结论:分离纯化得到了一种有微弱出血毒,具有较强抗凝活性的单一组分的酸性金属蛋白酶。BACKGROUND： A fibrinolytic enzyme （FLE） was purified from the ven- om of Agkistrodon blomhoffi brevicaudus by ion-exchange chromatography; meanwhile, it can dissolve thrombotic anti-thrombus drugs during onset of myocardial infarction and apoplexy. OBJECTIVE： To investigate the purification of FLE from the venom of Agkistrodon blomhoffi brevicaudus and research some biochemical and pharmacological characterizations. DESIGN： Controlled observational study. SETTING： Snake Venom Research Institute, Guangxi Medical University. MATERIALS： The experiment was carried out in Snake Venom Research Institute of Guangxi Medical University from July 2003 to June 2005. Kunming mice, weighing 18-25 g, of mean body mass of （20±2） g, aged 3- 4 months were provided by Experimental Animal Center of Guangxi Medical University. Agkistrodon blomhoffi brevicaudus was supplied by Snake Venom Research Institute of Guangxi Medical University. METHODS： FLE was purified with DEAE Sepharose CL-6B ion exchang columns and HiPrep Sephacryl S 100; purity and relative molecular mass were measured with high-effective chromatograph of liquid and polyacrylamidedel electrophoresis （PAGE）; isoelectric point was measured with isoelectric focusing electrophoresis; and then, components and partially pharmacological characteristics were also measured. MAIN OUTCOME MEASURES： Relative molecular mass, conformation of isoelectric point and amino acid, effect of inhibitor on FLE, hemorrhagic activity, and anticoagulant effect of FLE. RESULTS： All 30 rats and 12 rabbits were involved in the final analysis. Relative molecular mass was 59 100 and isoelectric point was 4.98. SDSPAGE acted as a zone in vitro. High-effective chromatograph of liquid showed a simple peak of FLE. This group belonged to metallo proteinases. Analysis of components of amino acid suggested that it contained a lot of acidity amino acids, had mild hemorrhagic toxicity and strongly thrombolytic effect. CONCLUSION： A simple acidity metallo proteinases, which is c

关 键 词：纤溶酶 蛇毒液类 分离和提纯 

分 类 号：R285[医药卫生—中药学]