小活络丸的免疫抑制、抗氧化及抗炎镇痛作用(英文)  

Immunosuppresive,antioxidative,anti-inflammatory and analgesic effect of Xiahuoluo pills

作　　者：潘竞锵 肖柳英 张丹[2] 韩超 林洁茹[2] 郑琳颖[2] 李博萍[2] 

机构地区：[1]广州市中医中药研究所,广东省广州市510130 [2]广州市中医医院药剂科,广东省广州市510130

出　　处：《中国临床康复》2006年第47期183-188,共6页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:小活络丸具有祛风除湿、活络通痹等功效,用于治疗风寒湿痹、肢体疼痛,麻木拘挛等症。目的:观察小活络丸对小鼠再次免疫应答、特异性免疫(包括细胞免疫和体液免疫)、非特异性免疫(包括补体C3、单核-巨噬细胞系统和红细胞黏附功能)和自由基损伤,以及对疼痛及多种炎症模型的药理作用。设计:随机对照,分层实验。单位:广州市中医中药研究所和广州市中医医院药剂科。材料:选用NIH小鼠和ICR小鼠共628只,鼠龄6～8周。小活络丸(成分:胆南星、制川乌、制草乌、地龙、乳香(制)等;广州陈李济药厂生产,生产批号:19980612)。兔抗小鼠IgG和补体C3抗血清试剂盒,由广州市医药卫生研究所药物室提供;超氧化物歧化酶活性和丙二醛含量测定试剂盒,南京建成生物工程研究所产品。方法:实验于1998-09/1999-12在广州市中医中药研究所药理研究室和广州市医药卫生研究所药物研究室动物室完成。①观察小活络丸抑制鸡红细胞诱导小鼠再次免疫应答作用:取ICR小鼠84只,雌雄各半,取出20只作为空白对照组;其余均腹腔注射环磷酰胺0.2g/kg,1次/d,1d。第4和12天,共2次腹膜内注射鸡红细胞诱导免疫增强病理模型探讨再次免疫应答。空白对照组(20只)腹腔注射等量生理盐水;动物于加强免疫后,按随机抽签法分为3组:环磷酰胺组20只(灌胃环磷酰胺40mg/kg),小活络丸组21只(灌胃小活络丸混悬液5.54g/kg),模型组20只(灌胃等量蒸馏水);均1次/d,连续灌胃给药7d。19d后分别按单向免疫扩散法测定血清IgG,补体C3含量,采用聚乙二醇沉淀法测定循环免疫复合物含量变化。②观察小活络丸抑制小鼠迟发型超敏反应作用:取ICR小鼠54只,雌雄各半。第1天皮下注射10g/L2,4-二硝基氟苯50μL/只致敏,第4天,将处理后动物按随机抽签法分为3组:泼尼松组(灌胃泼尼松0.01g/kg),小活络丸组(灌胃小活络丸混悬液5.54g/kgBACKGROUND： Xiaohuoluo pill can expel pathogenic wind, dampness and activate collaterals. It is used for treatment of Bi-syndrome due to wind-cold-dampness, pain and numbness in limbs. OBJECTIVE： To observe the pharmacological effect of Xiaohuoluo pills on secondary immune response, specific immunity （including cellular immunity and humoral immunity）, non-specific immunity [including complement 3（C3）, mononuclear phagocyte system （MPS） and red blood cell （RBC） adhesion function] and free radical injury as well as pain and many other inflammations in mice. DESIGN： A randomized controlled stratified trial. SETTING： Guangzhou Institute of Traditional Chinese Medicine and Chinese Materia Medica; Department of Pharmacy, Guangzhou Hospital of Traditional Chinese Medicine. MATERIALS： Totally 628 NIH and ICR mice of 6 to 8 weeks were involved in this trial. Xiaohuoluo pills （components： Dannanxing, Zhichuanwu, Zhicaowu, Dilong, Ruxiang and so on; Chenli Pharmaceutical Factory, Guangzhou; Batch No. 19980612） were used in this trial. Rabbit antimouse immunoglobulin G （IgG） and C3 antiserum reagent kit （Guangzhou Institute of Medicine and Health） and reagent kit for measuring the antioxidizing activity of superoxide dismutase （SOD） and the level of malondialdehyde （MDA） （Jiancheng Institute of Bioengineering, Nanjing） were used. METHODS： This trial was carried out in the Guangzhou Institute of Traditional Chinese Medicine and Chinese Materia Medica; Department of Pharmacy, Guangzhou Institute of Medicine and Health during September 1998 to December 1999. ① To observe the suppressive effect of Xiaohu.oluo pills on cock red blood cell （CRBC）-induced secondary immune response： Eight-four ICR mice, male and female in half, were selected. Twenty of 84 mice served as blank controls; The other 64 mice were intraperitoneally injected with cyclophos-phamide （CY） of 0.2 g/kg once. On the 4^th and 12^th days, CRBC was intraperitoneally injected into the mice twice to in

关 键 词：自身免疫疾病/中药疗法 小活络丹 免疫抑制 超氧化物歧化酶/血液 丙二醛/血液 镇痛 

分 类 号：R339.2[医药卫生—人体生理学]