吲哚美辛对阻塞性黄疸病人外周血淋巴细胞IL-10、IL-2活性的影响  

Effects of indometacin on activity of IL-10 and IL-2 in human peripheral lymphocytes of patients with obstructive jaundice

作　　者：李延光[1] 邹声泉[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院普通外科,武汉430030

出　　处：《腹部外科》2006年第6期365-366,共2页Journal of Abdominal Surgery

摘　　要：目的研究吲哚美辛对阻塞性黄疸病人外周血淋巴细胞IL-10、IL-2活性和血浆中前列腺素E2(PGE2)水平的影响。方法于阻塞性黄疸病人用药前及口服吲哚美辛(25mg,3次/d)7d后取周围血,分别用放射免疫和ELISA法测定其血浆中的PGE2水平及外周血淋巴细胞IL-10、IL-2活性。结果用药前,阻塞性黄疸病人血浆中PGE2水平及外周血淋巴细胞IL-10活性明显升高,而IL-2活性明显下降,且IL-10、IL-2的活性与PGE2水平显著相关;用药后,病人血淋巴细胞IL-2活性明显升高,而IL-10活性及血浆中PGE2水平明显下降,且与用药前相比有显著性差异。结论阻塞性黄疸病人存在细胞免疫功能受损,吲哚美辛能够明显改善其免疫功能状态,有可能成为临床上降低阻塞性黄疸病人易感性的有价值的药物之一。Objective To study the effects of indometacin on serum PGF4 and activities of IL-10 and IL-2 in human peripheral blood lymphocytes of patients with obstructive jaundice. Methods Before and 7 days after oral administration of indometacin（25 mg, 3 times every day）, peripheral blood was collected. By using radioimmunoassay and ELISA, the serum PGE2 and activities of IL-10 and IL- 2 in peripheral lymphocytes were determined, respectively. Results Before administration, serum PGE2 and ILl0 activity of blood lymphocytes in patients with obstructive jaundice were significantly increased, while IL-2 activity significantly reduced, and the activities of IL-10 and IL-2 were significantly correlated with serum PGE2. After administration of indometacin, the serum PGE2 and the IL-10 activity of blood lymphocytes and serum PGE2 were significantly decreased,and the IL-2 activity significantly increased. Conclusion Immune dysfunction in patients with obstructive jaundice may be the reason for high susceptibility to infection and indometacin might become a valuable drug for improving immune function and decreasing susceptibility to infection.

关 键 词：吲哚美辛 胆汁淤积 淋巴细胞 免疫活性 

分 类 号：R392.12[医药卫生—免疫学]