云南魔芋种质资源亲缘关系的ISSR分析  

ISSR Analyses of Relative Relationships among Germplasm Resources of Amorphophallus Blume from Yunnan Province

作　　者：滕彩珠[1] 刁英[1] 常福浩森[1] 谢世清[2] 韩永华[3] 胡中立[1] 

机构地区：[1]武汉大学发育生物学教育部重点实验室,湖北武汉430072 [2]云南农业大学魔芋研究所,云南昆明650201 [3]广西师范大学生命科学学院,广西桂林541004

出　　处：《安徽农学通报》2006年第11期54-56,共3页Anhui Agricultural Science Bulletin

基　　金：云南省财政厅综合开发办公室科技推广项目资助

摘　　要：用ISSR技术对30份魔芋种质资源进行了DNA多态性分析。从65个随机引物中筛选出11个扩增基因组DNA,共获116条带,其中102条为多态性标记,每个引物平均提供10.55个标记信息。UPMGA法聚类分析结果表明:这30份魔芋种质资源间的Jaccard相似系数都比较大,在0.65以上;甜魔芋和花魔芋以及西盟魔芋和珠芽魔芋的关系比较近;白魔芋的遗传多样性较为丰富,它与花魔芋的关系较近。The ISSR analysis was conducted in the 30 germplasm resources in Amorphophallus. Among 65 random primers, 11 were selected for amplification of the 30 genomic DNAs. Total 116 RAPD fragments were generated, 102 of them were polymorphic marks. Every primer offered 10, 55 marker bands on average. The molecular phylogenetic tree obtained by UP- MGA method showed that Jaccard similarity coefficient of the 30 germplasm resources is quit high, over 0. 65. Genetic diversity in Amorphophallus albus is richer than that of others, and it is close to Amorphophallus konjac. Amorphophallus dunnii is close to Amorphophallus konjac, while Amorphophallus krausei is close to Amorphophallus guripingensis.

关 键 词：魔芋 ISSR 多态性 亲缘关系 

分 类 号：S58[农业科学—作物学]