大蒜分生组织培养的染色体遗传稳定性研究  

Study on chromosome hereditary stability of the garlic tissues of dividing and growing when cultured

作　　者：张恩让[1] 任媛媛[1] 胡华群[1] 程智慧[2] 

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《山地农业生物学报》2006年第6期497-500,共4页Journal of Mountain Agriculture and Biology

基　　金：中德政府间合作项目资助(CH-01/04)

摘　　要：选取改良蒜、金堂早蒜、彭县晚熟、苍山蒜和欧引01等5个不同生态型大蒜的分生组织，在MS固体培养基上进行培养，并对培养的后代进行染色体倍性鉴定。结果表明，茎尖分生组织诱导出幼芽的细胞染色体二倍体率保持在96．7％-100．0％；增殖芽的细胞染色体二倍体率保持在93．7％-100．0％；生根阶段根尖的细胞染色体二倍体率保持在93．3％～100．0％；再生植株的染色体数日基本没有变异，二倍体率在93．7％～100．0％。Five different ecotypic garlic cultvars （gailiang garlic, Jintang early garlic, Pengxian late-maturing, Cangshan garlic and Europe 01 ） were choosed to catch the tissue of dividing and growing, it is cultured at the solid MS medium, and chromosome times are identified in later generation. The result shows that the number of chromosome does not change basically in bud which comes from tissues of dividing and growing in stem sharp, in proliferation stage of bud, in taking root stage and in recycling plants,the liploid rate is between 93. 7% and 100. 0%.

关 键 词：大蒜 分生组织 组织培养 细胞染色体 遗传稳定性 

分 类 号：S633.401[农业科学—蔬菜学]