树木溃疡病菌主要类群系统地位及茶藨子葡萄座腔菌的种内一致性  

Molecular Relationship of genus Botryosphaeria,genus Coniothyrium,genus Dothichiza and the Consistency of Botryosphaeria dothidea

作　　者：张星耀[1] 赵嘉平[1] 梁军[1] 吕全[1] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室,北京100091

出　　处：《林业科学》2006年第11期63-68,共6页Scientia Silvae Sinicae

基　　金：国家自然基金项目"树木溃疡病病原真菌类群遗传多样性与分子系统研究"(30271084)

摘　　要：报道利用微卫星引物[(GT)n、(CGA)n、(CCA)n、(GAA)n、(CAA)n、(GA)n]和小卫星引物M13进行的葡萄座腔菌属、盾壳霉属和疡壳孢属真菌的随机扩增微卫星多态性PCR(RAMs_PCR)遗传多样性结果:42个参试菌株被分为4个RAMs分类群;其中22个葡萄座腔菌属菌株和6个小穴壳菌属菌株分布于这4个类群中;RAMs分类群Ⅰ,Ⅱ,IV仅仅由葡萄座腔菌属和小穴壳菌属菌株构成,部分茶子葡萄座腔菌,4个贝伦格葡萄座腔菌和所有的盾壳霉属和疡壳孢属菌株构成了RAMs分类群Ⅲ。研究结果显示通过形态学确定的茶藨子葡萄座腔菌种内具有遗传不一致性,形态学上一致的28个茶子葡萄座腔菌菌株识别为4个不同的表观群;造成苹果轮纹病菌和苹果干腐病菌的贝伦格葡萄座腔菌菌株与部分茶子葡萄座腔菌在分子水平上非常相似;研究结果亦显示盾壳霉属与疡壳孢属真菌有着较近的系统关系。The fungi of genus Botryosphaerla, genus Coniothyrium and genus Dothichiza can cause huge disaster in forestry and man-made poplar forestry in China. Six micro-satellites primers [ （GT） n, （CGA） n, （CCA） n, （GAA） n, （CAA） n, （GA） n ] and one mini-satellites primer M13 used to identification the molecular relationship of those three genus. All 42 tested isolated isolates are clustered into 4 groups, 22 Botryosphaeria isolates and 6 Dothiorella isolates are disperse in this 4 groups, but group Ⅰ, Ⅱ and Ⅳ formed only by the Botryosphaeria and Dothiorella isolates. However, the other 6 Botryosphaeria dothidea isolates and 4 Botryosphaeria berengeriana isolates formed the subgroup of group m , 6 Coniothyrium isolates and 2 Dothichiza isolates only occurred in the subgroup of group m . The results of RAMs-PCR showed that the Botryosphaeria dothidea or Dothiorella isolates which identification by anamorph characters were not a monophyletic group. The putative pathogenic fungus of apple stem rot and ring spot, B. berengeriana and its physiological form could not formed a clear cluster branch separately and which merging with some B. dothidea isolates in a complex group. This result illustrated that Botryosphaeria berengeriana is very similarly with some B. dothidea isolates. The result also illustrated a close relationship between genus Coniothyrium and Dothichiza .

关 键 词：葡萄座腔菌属 盾壳霉属 疡壳孢属 茶蔗子葡萄座腔菌 随机扩增微卫星多态性PCR 树木溃疡病 

分 类 号：S718.81[农业科学—林学] Q939.5[生物学—微生物学]