RP-HPLC法测定川芎CO_2超临界流体萃取物中丁烯基苯酞的含量  

Determination of the content of butylidenephthalide in Rhizoma chuanxiong CO_2 supercritical fluid extracts by RP-HPLC

作　　者：侯晓虹[1] 李岩[1] 高艳 唐星[3] 吴群[3] 

机构地区：[1]沈阳药科大学制药工程学院,辽宁沈阳110016 [2]盘锦市药品检验所,辽宁盘锦124010 [3]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2006年第12期788-790,共3页Journal of Shenyang Pharmaceutical University

摘　　要：目的测定川芎二氧化碳超临界流体萃取物中丁烯基苯酞的含量。方法采用HPLC法。色谱条件为色谱柱：Diamonsil C18（200mm×4．6mm，5μm）；流动相：甲醇-体积分数为10％异丙醇水溶液（体积比为60：40）；流速：1．0mL·min^-1；检测波长：235nm；柱温：40℃。结果丁烯基苯酞在7．28～72．8μg·mL^-1内线性关系良好（r=0．9995），平均回收率为99．7％（RSD=2．72％，n=6）。3批样品中丁烯基苯酞的含量质量分数分别为4．86％（RSD=1．25％，n=3）、5．00％（RSD=1．87％，n=3）和4．81％（RSD=1．87％,n=3）。结论HPLC法可以测定川芎二氧化碳超临界流体萃取物中丁烯基苯酞的含量。供试品中丁烯基苯酞与其他组分的色谱峰分离度良好。Objective To determine the content of butylidenephthalide in Rhizoma chuanxiong CO2 supercritical fluid extracts by reversed-phase HPLC method. Methods The sample was separated on a C18 column with the mobile phase of methanol-10 % isopropanol in water solution（ V ： V = 60 ： 40）. The flow rate was 1.0 mL＇min-! and the detection wavelength was set at 235 nm. The column temperature was 40℃. Results The calibration curve was linear in the range of 7.28 -72.8μg· mL^-1 for butylidenphthalide（ r = 0. 999 5）. The average recovery was 99.7 % （RSD = 2.72 %, n = 6）. The contents of butyllidenephthalide in three batches of the Rhizoma chuanxiong CO2 supercritical fluid extracts were 4.86 % （RSD = 1.25 %, n = 3）,5.00% （RSD = 1.87%, n = 3）and 4.81% （RSD = 1.87%, n = 3）, respectively. Conclusions The method is simple and accurate, it can be used as a method of quality control for products containing butylidenephthalide.

关 键 词：川芎 二氧化碳超临界流体 丁烯基苯酞 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]