柳桉组培快繁技术  

Micropropagation of Eucalyptus saligna

作　　者：吴美华 林文革 兰贺胜 杨宗武[3] 李海花 陈水洲 

机构地区：[1]福建省永安林业(集团)股份有限公司种苗中心,福建永安366000 [2]福建省林木种苗总站,福建福州350003 [3]福建省林业科学研究院,福建福州350012

出　　处：《福建林业科技》2006年第4期159-162,共4页Journal of Fujian Forestry Science and Technology

基　　金：福建省林木种苗科技攻关项目"福建省桉树良种选育与扩繁应用"的部分研究内容

摘　　要：以3年生柳桉优树环割促萌枝条为外植体。以MS为基本培养基，通过激素种类与浓度、大量元索配比等试验，探讨了柳桉腋芽诱导、继代增殖、生根培养基配方。结果表明：外植体宜采用中等幼嫩的茎段，用75％酒精消毒30s、0．1％升汞溶液消毒4min效果最佳；在3／4MS＋0．3mg·L^-1 6-BA＋0．2mg·L^-1NAA培养基上进行侧芽诱导。柳桉外植体出芽率最高；在改良MS＋0．2mg·L^-1 6-BA＋0．2mg·L^-1 NAA继代培养基上，柳桉增殖效果最好，其芽体健壮，生长正常，增殖率3倍以上；应用1／2MS培养基附加ABT 1^# 0．5mg·L^-1和IBA0．1mg·L^-1，其生根率可达95％以上。生根3～4条。苗木生长健壮。移栽成活率可达92％以上。这些配方已成功地应用于柳桉优良无性系苗木的工厂化生产，单个超净台月苗木生产能力达7万株以上。A tissue culture protocol by shoot proliferation is developed for Eucalyptus saligna through adjusting the plant growth regulators and ratio of macro-elements using sprouts coUected from stubs of three-year-old plus trees as explants. The main results are as follows： 1. Half lignified shoots were most suitable for explants. Dipping them in solution of 75 % alcohol for 30 seconds and then in 0.1% HgCl2 for 4 minutes are the best way for their disinfection; 2.3/4 MS supplemented with 0.3 mg·L^-1 6-BA, 0.2 mg·L^-1 NAA and 20 g·L^-1 sugar is crucial for shoot germination and proliferation, while amended MS supplemented with 0.2 mg·L^-1 6-BA, 0.2 mg·L^-1 NAA and 30 g·L^-1 sugar for dump shoot induction; 3.1/2 MS supplemented with 0.5 mg·L^-1 ABT-1 and 0.1 mg·L^-1 IBA is suitable for rooting, with rooting rate up to 95 % ; and 4. The survival rate of out-planting in nursery is more than 92 %, The protocol has been successfully used in large-scale clonal production of the seedlings.

关 键 词：柳桉 组织培养 快繁技术 育苗 

分 类 号：S796[农业科学—林木遗传育种]