十四属蓝藻液泡的检查和分离  

Examination and Isolation of Vacuoles from 14-Genus Cyanobacteria

作　　者：宋文贞[1] 夏曦中[1] 郭厚良[1] 

机构地区：[1]武汉大学生命科学学院生物学实验教学中心,武汉430072

出　　处：《氨基酸和生物资源》2006年第4期18-20,24,共4页Amino Acids & Biotic Resources

基　　金：国家自然科学基金资助项目;编号39870083。

摘　　要：采用压片法,渗透冲击法和原生质球法,对14个属的不同蓝藻进行了液泡存在的检查和分离试验,每种蓝藻都检查到液泡,无一例外。其中鱼腥藻、念珠藻、项圈藻、席藻、简孢藻等都能形成液泡化原生质球,原生质球低渗破裂释放液泡,液泡得率极高,不同种的液泡都耐低渗。因此,本研究证明液泡在蓝藻中的存在是普遍的。The examination and isolation of vacuoles from 14 - genus cyanobacteria were carried out. The 14 genera were Anabaena, Nosfoc, Anabaenopsis, Phormidium, Cylindrospermun, Plectonemaboryanum, Scytonema, Spirulina , Oscillatoria , Lyngbya , Gloeocapsa , Syenchococcus , Synechocystis and Microcystis. All the cyanobacteria had been found to have vacuoles. In the genera Anabaena, Noatoc, Anabaenopsis, Phormidium and Cylindrospernum the cells were prepared into the vacuolated spheroplasts, and vacuoles released by hypotonic treatment at a great rate.

关 键 词：蓝藻 液泡 

分 类 号：Q949.220.6[生物学—植物学]