实验细胞资源同功酶分析鉴定  

Identification of Experimental Cell Resources By Isoenzyme Analysis

作　　者：陈丽婷[1] 张雪瑾[1] 沈超[1] 郑从义[1] 

机构地区：[1]武汉大学生命科学学院,武汉430072

出　　处：《氨基酸和生物资源》2006年第4期21-24,共4页Amino Acids & Biotic Resources

基　　金：国家科技条件平台资金资助(项目号:2004DKA305400-4)

摘　　要：琼脂糖凝胶电泳检测细胞同功酶的方法,可用于实验细胞系种间鉴别和交叉污染检测。该方法所需样品少,时间短,费用低,并且具有较高的灵敏度。本文对HeLa细胞进行了LD、MD、G6PD同功酶检测灵敏度分析,结果表明:HeLa细胞LD和MD同功酶在细胞为5×107个/L时可有效检测出来,而G6PD分析时细胞为109个/L。20种实验细胞的LD、MD同功酶检测证明,每种细胞种属来源清楚,且均未被其它细胞污染。The agarose gel electrophoresis was applied for the species identification and pollution examination by analyzing the experimental cell isoenzymes. This method is convenient, inexpensive, sensitive and using only a small amount of sample. The sensitivity was analyzed by using the three groups of isoenzymes containing LD, MD, G6PD in HeLa cells. The result showed that the LD, MD isoenzymes could be obtained when the cell concentration beyound 5 × 10^4cell mL^-l, and 10^6cell mL^-1 were used for G6PD. The analysis of the LD, MD isoenzyme in 20 species of cells showed that each of them had actual species origin, and they were not polluted by other species of cells.

关 键 词：实验细胞资源 同功酶 琼脂糖凝胶电泳 

分 类 号：Q55[生物学—生物化学]