复合诱变选育林肯霉素高产菌株  

Compound Mutagenesis and Screening of High Productivity Strains of Lincomycin

作　　者：丁琳[1] 安志东（指导教师）[1] 

机构地区：[1]武汉大学生命科学学院生物学实验教学中心,武汉430072

出　　处：《氨基酸和生物资源》2006年第4期68-70,共3页Amino Acids & Biotic Resources

摘　　要：以林肯链霉菌9502(Streptomyces lincolnensis9502)为出发菌株,进行NTG诱变处理,并用高效的琼脂块培养法对菌株进行筛选,得到产林肯霉素相对效价提高35.4%的变异株9502-7。对9502-7菌株孢子采用紫外线处理,得到变异高产菌株9502-7-12,其相对效价较出发菌株提高50%以上。Streptomyces lincolnensis 9502, the starting strain in the experiment, was treated with NTG and mutant 9502 - 7 was screened by efficient agar piece cultural method. The mutant showed lincomycin - producing ability 35.4% higher than the starting strain. Then by treating the spores of mutant 9502 - 7 with UV, a strain of 9502 -7 - 12 was obtained, whose titer was increased 50% more than the strain 9502.

关 键 词：林肯链霉菌 林肯霉素 NTG诱变 紫外诱变 

分 类 号：Q93[生物学—微生物学]