流感嗜血杆菌氨苄西林耐药基因研究  

Detection of ampicillin resistant genes in Haemophilus in fluenzae

作　　者：张泓[1] 吴文娟[2] 李万华[1] 孔青[1] 盖晶[1] 陆权[1] 

机构地区：[1]上海交通大学附属儿童医院,200040 [2]上海交通大学医学院附属瑞金医院

出　　处：《中国感染与化疗杂志》2006年第6期377-379,共3页Chinese Journal of Infection and Chemotherapy

摘　　要：目的明确我院分离的流感嗜血杆菌(HI)氨苄西林耐药的基因。方法用 E 试验测定我院上呼吸道感染患儿鼻咽部分离300株 HI 对氨苄西林耐药情况;以 Nitrocefin 纸片检测β内酰胺酶;PCR 扩增及序列分析确定产酶株的基因型。结果31株氨苄西林耐药株均产β内酰胺酶,占总菌株数11%(32/300),PCR 检测出 TEM-1 31株,ROB-1 1株。结论产β内酰胺酶是 HI 对氨苄西林耐药的重要机制,TEM-1型是β内酰胺酶主要基因型,ROB-1型β内酰胺酶也首次被检出,值得关注和长期监测。Objective To study the mechanism of ampicillin resistance in Haemophilus influenza （HI） strains. Methods A total of 300 strains of HI isolated from children with respiratory tract infection were collected. Their resistance to ampicillin was detected by E-test. β-lactamase production was determined by chromogenic cephalosporin （nitrocefin） test. TEM1 and ROB-1 gene conferring ampicillin resistance were detected by polymerase chain reaction （PCR）. Results β- lactamase was produced in all the 32 ampicillin-resistant HI strains. TEM1 gene was identified in 31 of tile 32 anapicillin-resistant strains. ROB1 gene was detected in only 1 ampicillin-resistant strain, Conclusions TEM-1 β-lactamase is the major mechanism of anapicillin resistance in HI isolated from children with respiratory tract infection. ROB-1 gene should be carefully monitored for a long time.

关 键 词：流感嗜血杆菌 氨苄西林 耐药性 Β内酰胺酶 TEM-1基因 ROB-1基因 

分 类 号：R450[医药卫生—治疗学]