肝纤维化肝组织中肾上腺素能受体的表达研究  

EXPRESSION OF ADRENERGIC RECEPTORS IN LIVER TISSUE OF FIBROSIS

作　　者：高啸[1] 张芳杰[1] 晏维[1] 郭燕[1] 唐望先[1] 

机构地区：[1]华中科技大学同济医学院同济医院肝病研究所,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2006年第6期598-602,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金课题专项基金(30440088)

摘　　要：目的探讨肝纤维化大鼠肝脏组织中交感神经递质受体表达的变化情况。方法应用四氯化碳(CCl4)诱导大鼠慢性肝纤维化模型。23只Wistar大鼠随机分为正常对照组(N组)和肝纤维化模型组(M组)。应用逆转录聚合酶链反应(RT-PCR)和Westernblot方法检测肝脏组织中肾上腺素能受体mRNA及蛋白质的表达情况。结果肝纤维化大鼠肝脏组织中肾上腺素能受体mRNA和蛋白质表达均比正常组明显增加(P<0·01)。结论肝纤维化时,大鼠肝脏组织中肾上腺素能受体α1-AR和β2-AR表达增加,这可能是肝纤维化时交感神经促进肝纤维化发展的作用机制之一。Objective To investigate the expression of neurotransmitter receptors in rat liver tissue of fibrosis. Methods We used CCl4 to induce the model of liver fibrosis in 23 rats, which were randomly divided into the control group and the model group. Then we used RT-PCR and Western-blot to examine the mRNA and protein expression of neurotransmitter receptors. Results The expression of neurotransmitter receptors in the model group was much higher than in the control group （P〈0.01）. Conclusion The expression of adrenergic receptors α1-AR and β2-AR are increased in liver tissue of fibrosis, which may be the mechanism of sympathetic nerves promoting the development of liver fibrosis in rats.

关 键 词：纤维化 肾上腺素能受体 WESTERNBLOT MRNA表达 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学]