慢性乙型肝炎肝组织中MMP-2、TIMP-1与肝纤维化关系的研究  

Relationship between fibrosis and MMP-2/TIMP-1 in liver tissues from patients with chronic hepatitis B.

作　　者：陈超丽[1] 甄真[2] 刘晓平[1] 冯翠玲[1] 张新元[1] 

机构地区：[1]石家庄市第五医院肝病临床中心科,河北石家庄050021 [2]河北医科大学第三医院感染科,石家庄050051

出　　处：《临床肝胆病杂志》2006年第6期419-421,共3页Journal of Clinical Hepatology

摘　　要：为了研究慢性乙型肝炎患者肝纤维化与肝组织中基质金属蛋白酶-2(MMP-2)及其天然抑制物金属蛋白酶组织抑制剂-1(TIMP-1)的关系,对60例慢性乙型肝炎患者进行肝组织病理活检,其中S_110例,S_224例,S_312例,S_414例。采用苦叶酸天狼猩红染色检测胶原面积百分比,单克隆抗体(McAb)免疫组织化学染色检测MMP-2、TIMP-1阳性细胞的方法进行病理分析。结果显示：慢性乙型肝炎肝组织中胶原面积百分比与肝组织纤维化分期正相关(r=0．885,P=0．000)；慢性乙型肝炎肝组织中MMP-2与肝纤维化的分期无关(r=0．034,P= 0．896)；慢性乙型肝炎肝组织中TIMP-1和肝纤维化的分期正相关(r=0．760,P=0．000)；慢性乙型肝炎肝组织中MMP-2/TIMP-1和肝纤维化分期负相关(r=-0．674,P=0．000)。总之,TIMP-1参与了慢性乙型肝炎肝纤维化纤维化的过程,而MMP-2/TIMP-1是诊断肝纤维化的比较合适的指标。To investigate the relationship between liver fibrosis with matrix metalloproteinases -2 （ MMP -2） and their specific inhibitor - 1 （TIMP - 1 ）. The hepatic biopsy from 60 cases with chronic hepatitis B （ CHB ） was analyzed. Among these, 10 cases were S1 , 24 cases S2, 12 cases S3, 14 cases S4. The percentage area of collagen by Sirius red stain was analyzed. And MMP - 2, TIMP - 1 positive cells were detected in paraffin - embeded liver tissue from biopsy cases with CHB by immunohistochemical method using specific mono clonal antibody （McAb）. There was positive correlation between the percentage of collagen area and staging of liver fibrosis （ r = 0. 885, P = 0. 000 ）. There was no correlation between MMP - 2 and staging of liver fibrosis （ r = 0. 034, P = 0. 869）. There was positive correlation between TIMP - 1 and staging of liver fibrosis （r =0. 760, P =0. 000）. There was negative correlation between MMP -2/TIMP - 1 and staging of liver fibrosis （r = -0. 647 ,P = 0. 000）. It can be concluded that TIMP- 1 is involved in liver fibrosis, and MMP -2/TIMP - 1 is a proper marker for liver fibrosis in liver tissues of patients with CHB.

关 键 词：肝炎 乙型 慢性 肝纤维化 基质金属蛋白酶-2 金属蛋白酶组织抑制剂-1 

分 类 号：R512.62[医药卫生—内科学]