钙调素对钙调素结合蛋白-10和玉米非特异性脂转移蛋白与脂质结合活性的影响  

The Effects of Calmodulin on the Lipid-Binding Activity of CaM-Binding Protein-10 and Maize Non-specific Lipid Transfer Protein

作　　者：谢万钦[1] 赵立青[1] 白文艳[1] 李振鹏[1] 赵玉龙[1] 李翠凤[1] 

机构地区：[1]南开大学生命科学学院生物化学与分子生物学系,天津300071

出　　处：《植物生理与分子生物学学报》2006年第6期679-684,共6页Journal Of Plant Physiology and Molecular Biology

基　　金：国家自然科学基金项目(No.30370733)资助。~~

摘　　要：荧光标记的脂质结合实验表明,钙调素结合蛋白-10(CaMBP-10)具有典型的植物非特异性脂质转移蛋白与脂质结合的特性。进一步实验研究了钙调素(calmodulin,CaM)对CaMBP-10和玉米nsLTP与脂质结合的活性的影响,结果显示无论在有钙和无钙条件下,CaM对两者的影响均有不同之处,W-7和TFP能消除CaM的影响。提示CaM不仅与CaMBP-10和玉米nsLTP特异性相互作用,而且对2种脂转移蛋白可能具有不同的调节机制。Fluorescence-marked lipid binding experiment shows that CaMBP-10 has typical lipid binding feature of non-specific lipid transfer protein （nsLTP） （Figs. 1-3）. We have compared the effects of calmodulin （CAM） on the lipid-binding activity of CaMBP-10 and maize nsLTP. Different influences were found in the presence of either Ca^2＋ or EGTA （Figs.4a and 5a）. W-7 and TFP could abolish the influence of CaM （Figs.4b and 5b）. Therefore, it is suggested that CaM could interact specifically with both CaMBP-10 and maize nsLTP. Probably, there are different CaM regulatory mechanisms between CaMBP-10 and maize nsLTE

关 键 词：钙调素 脂转移蛋白 钙调素结合蛋白-10 脂质结合活性 

分 类 号：Q946[生物学—植物学]