嗅鞘细胞条件培养液对骨髓基质干细胞的诱导分化作用研究  

Study of the differentiation of BMSCs induced by the OECs conditioned medium

作　　者：冯林杰[1] 罗卓荆[1] 王晓礽[2] 沈学锋[2] 孟浩[1] 王亮[1] 史晓娟[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032 [2]第四军医大学神经生物学教研室

出　　处：《中国矫形外科杂志》2006年第24期1894-1895,I0004,共3页Orthopedic Journal of China

基　　金：国家863课题项目(2002AA216101)

摘　　要：[目的]探讨大鼠嗅神经鞘细胞条件培养液对同种大鼠骨髓基质干细胞的诱导分化作用。[方法]通过差速贴壁法获得较高纯度的嗅鞘细胞，在接种培养后的9～12d，收集并制备嗅鞘细胞条件培养液，与纯化培养第3代的骨髓基质干细胞共培养，观察其诱导分化后的形态学变化，免疫荧光染色鉴定诱导分化结果。[结果]发现大鼠嗅鞘细胞条件培养液对同种大鼠骨髓基质干细胞有明显的诱导分化作用，在72h时荧光显微镜下观察并计算MAP-2和GFAP阳性细胞的比率分别为55％、23％。[结论]大鼠嗅鞘细胞条件培养液有明显的诱导同种大鼠骨髓基质干细胞向神经样细胞分化的作用。[ Objective] To explore the differentiation of bone marrow stromal cells （BMSCs） induced by OECs conditioned medium. [ Method] The purified OECs were acquired by seleclive culture medium and cultivated in the polylysine coated plate. After 9 -12 days culture, OECs conditioned medium was obtained by 2000 r/min configuration and then co-cultivated with purified BM SCs which were 3th generation. The differentiation of BMSCs were observed under inverted microscope and were identified by immu- mofluorescence method. [ Result] The OECs conditioned medium had obviously effects on BMSCs＇differentiation . BMSCs differentiation into neuron-like cells ratio was 55% and differentiation into astroctes cells ratio was 23%. [ Conclusion] The conditioned medium which comes from OECs culture supernatant has obviously effects on BMSCs＇differentiation into neuron-like cells.

关 键 词：骨髓基质干细胞 嗅鞘细胞 共培养 分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]