骨髓间充质干细胞及其复合物修复兔桡骨缺损的实验研究  

Experimental study of rabbit segmental radial defects repaired by mesenchymal stem cells composite grafts

作　　者：李明[1] 曹豫江[1] 张德文[1] 罗聪[1] 倪滨[1] 江标[1] 

机构地区：[1]重庆医科大学附属儿童医院骨科,重庆400014

出　　处：《中国矫形外科杂志》2006年第24期1896-1899,1902,共5页Orthopedic Journal of China

基　　金：重庆市人事局归国人员资助课题(项目编号:渝人[2005]88号)

摘　　要：[目的]通过新西兰大白兔骨髓间充质干细胞离体培养后与去抗原牛松质骨复合植入新西兰大白兔桡骨缺损处,比较修复节段性桡骨缺损的效果,初步探讨治疗骨缺损的组织工程学方法。[方法]新西兰大白兔骨髓间充质干细胞(MSCs)离体培养、纯化、扩增后在等同于细胞培养的条件下与去抗原牛松质骨(BCB)复合培养,制成MSCs/BCB复合物并经电镜扫描证实复合物有细胞生长。制成同种异体新西兰大白兔桡骨干15mm节段性动物模型,MSCs/BCB复合物通过手术移植入动物模型桡骨缺损处,通过X线放射学、组织学、生物力学检测对比实验组与对照组、空白组骨缺损修复情况。[结果]术后2、4、8、12、16周实验组与实验对照组放射学检查评价新骨生成有显著性差异(P<0.01)。术后组织学检查新骨生成速度、生成量均有显著性差异(P<0.01)。16周生物力学三点抗弯曲实验载荷、弯曲应力检测,实验组与实验对照组新骨生成有显著性差异(P<0.01),实验组标本与正常基本一致。空白对照组各时点均无新骨形成,最后缺损由纤维组织充填。[结论]骨髓间充质干细胞(MSCs)是骨组织工程中适宜的种子细胞。去抗原牛松质骨能够与兔骨髓间充质干细胞体外复合培养,移植入异体新西兰大白兔后未见明显免疫排斥反应,是可以选择的细胞载体。MSCs/BCB复合物植入修复兔桡骨缺损能够加速新骨形成,修复效果明显优于单纯BCB植入。[ Objective] To discuss the tissue engineering method of treating bone defects through rabbit bone marrow mesenchymal stem cells（MSCs） culture in vitro and make composite grafts with anti -extracted bovine cancellous bone（BCB）. The composite grafts were implanted into segmental radial defects in rabbits was compared on the curative effect of repairing segmental bone defects in rabbits with the composite grafts. [ Method] The purified, culture -expanded MSCs were combined with BCB in vitro according to condition of cell culture. The composite grafts were tested by scanning electron microscope. An bone defect （15mm in length） was created at two radii in each rabbit. The composite grafts were implanted into segmental radial defects in rabbits through open operation. The curative effect was evaluated by radiographic examination, histologic analysis, biomechanical test after surgery in experimental groups and control＇s and blank＇s. [ Result] Roentgenographically, the bone defects that had been treated with grafts exhibited new bone formation increased with time. But the result of experimental groups were apparently superior to that of the control groups＇s at 2,4,8,12,16 week after operation（P 〈0.01 ）. The rate and quality of new bone formation were significantly different in the experimental and the control groups on histologic analysis（ P 〈0.01 ）. The mechanical strength of composite grafts reached almost normal level at 16 week. the loads and bending - stresses of experimental groups were remarkably higher than those of the control groups. In the defects in blank groups, no formation of new bone were found after operation and were finally repaired only by fibrous tissue. [ Conclusion] MSCs are ideal seeded cells for bone tissue engineering. BCB/MSCs may be compounded in culture. The composite grafts cause no obvious immune rejection after being transplanted into allogenic rabbit. BCB may be used as an alternative carrier for seeded cells. These studies demonstrate that the composite gra

关 键 词：骨髓间充质干细胞 去抗原牛松质骨 细胞培养 骨移植 骨缺损 

分 类 号：R687[医药卫生—骨科学]