影响水稻籼粳亚种间杂交构建回交自交系的花药培养效果的2个因素  

Two Factors Influencing on Anther-cultural Effect of Backcross Inbred Lines Constructed by Offspring of Oryza sativa ssp.Indica×Japonica

作　　者：向珣朝[1] 张楷正[2] 李季航[2] 李平[2] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]四川农业大学水稻研究所

出　　处：《植物生理学通讯》2006年第6期1041-1044,共4页Plant Physiology Communications

基　　金：国家"863"计划(2003AA212030);教育部长江学者和创新团队发展计划(IRT0453)

摘　　要：水稻籼粳亚种间杂交构建的回交自交系(BIL)的10个株系在3种诱导培养基、1种分化培养基以及样株剪叶与否处理的结果表明,通用培养基(GM)不适合于籼粳交后代BIL的花药培养。N6和SK3都有较高的出愈率,但N6的绿苗发生率比SK3高,说明N6的愈伤组织质量和诱导效果更优于SK3。无论N6还是SK3,剪去样株叶片的绿苗发生率显著下降,显示花药培养过程中绿叶的作用很重要。完善籼粳亚种间杂交构建BIL的花药培养体系和从较多的株系中取材可保证花药培养的后代有较大的二倍体群体以供选择。Ten lines of backcross inbred lines （BIL） constructed by offspring of Oryza sativa ssp. IndicaxJaponica were treated in three induction media, a differentiation medium, cutting leaves or not of sample plants. Results showed that, general medium （GM） was not fit to anther culture of BIL. N6 and SK3 both hada high callus induction frequency, but the proportion of green plantlet differentiation of N6 was higher than that of SK3. It suggested that callus quality and induction effect of N6 was better. The proportion of green plantlet differentiations was decreased evidently when cutting leaves were done in N6 or SK3. It indicated that green leaves could have very important effect during the course of anther culture. To perfect the anther-cultural system of BIL and select more cultured materials from BIL, there would be more diploid to be selected in anther-cultural progeny.

关 键 词：水稻籼粳杂交后代 回交自交系 花药培养体系 

分 类 号：S511.2[农业科学—作物学]