烟草黄酮醇合成酶基因的克隆及其序列分析  

Cloning and Sequence Analysis of Flavonol Synthase Gene from Tobacco (Nicotiana tabacun L.)

作　　者：黄伟伟[1] 杨曦[1] 张常娥[1] 方斌[1] 马靓[1] 常俊丽[1] 杨广笑[1] 何光源[1] 

机构地区：[1]华中科技大学中英HUST-RRes基因工程和基因组学联合实验室,武汉430074

出　　处：《植物生理学通讯》2006年第6期1059-1062,共4页Plant Physiology Communications

基　　金：国家"973"项目(2002CB111302)子课题;国家自然科学基金(30370807)

摘　　要：根据已知的黄酮醇合成酶cDNA保守序列设计引物,用RT-PCR技术从烟草叶片中扩增获得黄酮醇合成酶cDNA片段,再用RACE方法得到其两端序列。根据获得的序列,设计引物分离得到完整的1188bp的黄酮醇合成酶基因,其开放阅读框编码346个氨基酸。序列分析显示,烟草黄酮醇合成酶与高杯花、矮牵牛和马铃薯的同源性分别为87%、86%和84%,与其它物种中的同源性也在80%左右,表明不同物种中黄酮醇合成酶基因具有高度同源性。此外,在氨基酸水平上,该酶与其它依赖于2-酮戊二酸的双加氧酶及其相关酶也具有同源性。According to the conserved cDNA sequence of flavonol synthase （FLS） gene, a pair of primers was designed, and FLS was cloned from tobacco （Nicotiana tabacun L.） leaves using RT-PCR and RACE. The full length cDNA sequence was 1 188bp and it included a open reading frame （ORF） which encoded 346 residues. Sequence analysis showed that homologies of FLS cDNA from tobacco with Nierembergia caernlea, Petunia hybrida and Solanum tuberosum were 87%, 86% and 84%, and which among the others were about 80%. The results indicated that FLS was highly stable in evolution of plants. On the other hand, it also had similarity with other 2-oxoglutarate-dependent dioxygenases on amino acids level.

关 键 词：烟草 黄酮醇合成酶基因 RACE 序列分析 

分 类 号：Q943.2[生物学—植物学]