杂交方法获得的拟南芥蔗糖转运蛋白基因SUC3/SUC5双突变体及其PCR鉴定  

Acquired Sucrose Transporter Gene SUC3/SUC5 Double-mutant of Arabidopsis thaliana by Cross and Its PCR Identification

作　　者：吴晓丹[1] 张立军[1] 白雪梅[1] 李敏[1] 阮燕晔[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,沈阳110161

出　　处：《植物生理学通讯》2006年第6期1073-1076,共4页Plant Physiology Communications

摘　　要：文章以拟南芥T-DNA插入纯合突变体atsuc3和atsuc5为亲本,采用传统杂交技术与PCR鉴定相结合的方法获得了atsuc3/atsuc5纯合双突变体。In this research, we crossed T-DNA inserted mutants atsuc5 with atsuc3 to produce atsuc3/atsuc5 double-mutant and used PCR to identify homogeneous double-mutant. In addition, the problems of cross and PCR process were discussed.

关 键 词：拟南芥 atsuc3/atsuc5双突变体 杂交 PCR 

分 类 号：Q943[生物学—植物学]