荔枝离体培养物DNA提取方法的研究  

Study on methods of extracting DNA of lichee isolated culture

作　　者：黄素华[1] 赖钟雄[1] 

机构地区：[1]福建农林大学亚热带果树研究所

出　　处：《河北建筑科技学院学报》2006年第4期39-41,共3页Journal of Hebei Institute of Architectural Science & Technology

基　　金：国家自然科学基金(39870544);霍英东教育基金会高等青年教师基金(71026)

摘　　要：采用十二烷基硫酸钠(SDS)并补加非水溶性聚乙烯吡咯烷酮法,提取荔枝胚性培养物基因组DNA,有效地去除了植物组织中所含的多酚、单宁、色素及多糖类物质,得到高质量的荔枝胚性培养物基因组DNA。此DNA适合作为PCR模板应用,能较好地用于RAPD分析,这是一种简便、快捷、经济的荔枝胚性培养物基因组DNA提取的方法。The DNA of lichee isolated culture was extracted by lauryl sodium sulfate and no-water-solution polyethylene pyrrolidone, which can effectively wipe off the polyphenol, tannin, pigment and other polyoses, and can provide hige quality DNA of lichee isolated culture which adopt to be as PCR cyclostyle to analyze RAPD. It is convenient and economic to extract DNA of lichee isolated culture.

关 键 词：荔枝 离体培养物 DNA提取 RAPD 

分 类 号：S667.1[农业科学—果树学]