灵芝孢子粉对雄性糖尿病大鼠睾丸细胞色素C、线粒体钙影响的研究  

Effects of Ganoderma Lucidum Spores on Cytochrome C and Mitochondrial Calcium in the Testis of NIDDM Rats

作　　者：王柏欣[1] 王淑秋[1] 秦文波[2] 王淑湘[1] 马小茹[1] 张婷[1] 

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007 [2]佳木斯大学附属第一医院泌尿外科,黑龙江佳木斯154007

出　　处：《中华男科学杂志》2006年第12期1072-1075,共4页National Journal of Andrology

基　　金：黑龙江省卫生厅医学科研基金(2003-171)

摘　　要：目的:观察灵芝孢子对2型糖尿病大鼠睾丸组织中细胞色素C(Cyt-C)及线粒体钙的影响。方法:Wistar雄性大鼠50只,随机分成3组(对照组、模型组、灵芝孢子粉组)。模型组及灵芝孢子粉组大鼠经尾静脉一次性按25mg/kg体重注射2%链脲佐菌素(STZ),对照组尾静脉注射等量枸橼酸缓冲液。正常饮食喂养2周后,进行糖耐量试验,模型组和灵芝孢子粉组以糖耐量异常者保留,余去除,并改喂高脂高糖饮食,灵芝孢子粉组另加灵芝孢子粉[250mg/(kg·d)]持续10周,实验结束前1天做糖耐量试验,取双侧睾丸检测相关指标。结果:模型组睾丸线粒体Cyt-C、线粒体钙均显著低于对照组和灵芝孢子粉组(P<0.05),胞质Cyt-C显著高于对照组和灵芝孢子粉组(P<0.05)。结论:Cyt-C、钙离子参与2型糖尿病大鼠睾丸组织损伤,灵芝孢子粉对糖尿病大鼠睾丸组织具有保护作用。Objective： To observe the effects of Ganoderma lucidum spores on Cytochrome C （Cyt-C） and mitochondrial calcium in the testis of NIDDM rats. Methods： Fifty male Wistar rats were divided randomly into three groups： model, ganoderma and normal control, the first two groups injected with 2% STZ through vena caudalis, and the last one with half-and-half sodium citrate/citrate buffer solution. Two weeks after normal diet, glucose tolerance tests were performed and the rats with abnormal glucose tolerance from the model and ganoderma groups received high-fat and high-carbohydrate food, the ganoderma group given Ganoderma lucidum spores （250mg/[ kg ~ d] ） in addition, both for 10 weeks. Glucose tolerance tests were repeated 1 day before the end of the experiment and the rats were castrated and relevant indexes measured. Results： The NIDDM model was successfully constructed. In the model group, the levels of mitochondrial Cyt-C and mitochondrial calcium were significantly lower （P 〈 0.05 ） while that of the plasma Cyt-C was significantly higher than in the ganoderma and the control groups, Conclusion ： Cyt-C and calcium ion are involved in the damage of the testis. Ganoderma lucidum spores can protect the testis of NIDDM rats.

关 键 词：灵芝孢子 2型糖尿病 细胞色素C 线粒体钙 睾丸 大鼠 

分 类 号：R285[医药卫生—中药学]