遗传工程稻叶绿体psbA基因的克隆及结构分析  

Isolation and Characterization of the Chloroplast psbA Gene from Engineering Rice

作　　者：钟珍萍[1,2] 吴乃虎[1,2] 

机构地区：[1]福建农业大学作物科学学院 [2]中国科学院发育生物学研究所

出　　处：《福建农业大学学报》1996年第4期387-393,共7页Journal of Fujian Agricultural University

摘　　要：分别制备了遗传工程稻ＧＥＲ－１及其ＤＮＡ供体（慈利玉米）、ＤＮＡ受体（水稻湘早籼８号）的叶绿体ＤＮＡ（ｃｐＤＮＡ），对三者之间的多种限制性内切酶酶切图谱比较表明，ＧＥＲ－１的ｃｐＤＮＡ基本与ＤＮＡ受体水稻一致．进一步克隆了ＣＥＲ－１的ｐｓｂＡ基因，并构建其酶切图谱、测定其３′非翻译区（３′ＵＴＲ）序列，初步认为该基因的序列结构与水稻相同，从而排除了ＧＥＲ－１的性状变异是由其ｐｓｂＡ基因发生变化所致的可能．此外。Chloroplast DNA(cpDNA) of engineering rice (GER 1), its DNA donor(maize) and its DNA acceptor (rice) were digested with several restriction enzymes respectively. Moreover the cloning and characterization of GER 1 psbA gene were carried out. These results indicate that the structures of GER 1 and rice chloroplast DNA, especially for their 2 200 bp Eco RI fragment containing psbA gene,are very similar. So it is impossible for GER 1 that its inheritable vatiations result from the mutation of psbA gene.

关 键 词：遗传工程稻 叶绿体 PSBA 克隆 水稻 

分 类 号：Q78[生物学—分子生物学]