用分群分析法（BSA）鉴别与秋稻品种Aus373广亲和基因连锁的RFLP标记  

Identification of RFLP Markers Linked with Wide Compatibility Gene in Variety Aus373 of Ecotype Aus of Rice by Using Bulked Segregant Analysis

作　　者：陈忠明[1,2,3] 邹江石[1,2,3] 朱立煌 李和标[1,2,3] 谷福林 顾铭洪[1,2,3] 

机构地区：[1]扬州大学农学院农学系 [2]江苏省农业科学院粮食作物研究所 [3]中国科学院遗传研究所

出　　处：《江苏农业学报》1996年第4期1-6,共6页Jiangsu Journal of Agricultural Sciences

摘　　要：Ａｕｓ３７３是典型的秋稻广亲和品种。以Ａｕｓ３７３／轮回４２２Ｆ２群体为基础，应用分群分析法（ＢＳＡ）建立可育池和半不育池。通过对两池的ＲＦＬＰ分析，发现与育性共分离的两个染色体区段。进一步通过对分离群体ＲＦＬＰ分析，证实了轮回４２２广亲和基因为Ｓ５ｎ，与分子标记ＲＧ２１３、Ｇ２００连锁，并发现Ａｕｓ３７３广亲和基因位于第七染色体上，与分子标记ＲＺ４８８、ＲＧ５１１连锁。Aus373 is a typical wide compatibility variety in ecotype Aus. Based on the F 2 population of hybrid Aus 373/Lunhui 422, fertile and semisterile pools were set up by bulked segregant analysis. The RFLP analysis of the two pools led to the discovery of two chromosome segments co segregating with fertility. The RFLP analysis of the segregating population further approved that Lunhui422 contained the wide compatibility gene S 5 n linked with molecular markers RG213 and G200. We also found that the wide compatibility gene of Aus373 was located on chromosome 7, linked with molecular markers RZ488 and RG511.The gene was temporarily designated S a(t) n.

关 键 词：杂交稻 广亲和基因 RFLP BSA 

分 类 号：S511.032[农业科学—作物学]