离子束注入硅酸盐细菌诱变选育研究  

Breeding of Silicate Bacteria by Ion Implantation

作　　者：卢文轩[1] 蒋立科[1] 罗曼[2] 

机构地区：[1]安徽农业大学生命科学学院 [2]暨南大学生物医学工程研究所,广州510632

出　　处：《华中农业大学学报》2006年第6期636-639,共4页Journal of Huazhong Agricultural University

基　　金：国家"863"计划项目(2003AA241171)资助

摘　　要：硅酸盐细菌能分解铝硅酸盐类矿物中的钾、磷、硅等元素,直接供植物生长利用。采用低能N离子束注入技术对硅酸盐细菌进行诱变,然后经有氮培养基初筛、缺钾培养基复筛,获得解钾能力强的硅酸盐细菌S-05突变株,扫描电镜观察菌株的形态。进行盆栽试验,研究S-05菌株对水稻根际土壤速效钾含量的影响。结果发现,发酵液50倍稀释处理组速效钾含量最高,比原菌株处理组(CK1)和空白对照组(CK2)分别提高55.79%和260.84%。K, P and Si can be released from soil mineral by silicate bacteria , then were absorbed by plants directly. By low-power N ion implantation under dose from 5. 2 × 10^14 to 18. 2 × 10^14 N^＋/cm^2 （ 10 keV ）, sixteen silicate bacteria mutants were screened out firstly under azotic medium, then strain S-05 was sreened by roseite culture. Morphologic observation of S-05 and LTF-22 cell were conducted with SEM. Tb, e effect of strains S-05 and LTF-22 on the content of available K in rice rhizosphere was also studied by pot culture test. It has been found that the content of available K in rice rhizosphere treated with S-05, which diluted to 1 ： 50, increased 55.79% and 260. 84% respectively, compared with CK1 and CK2 .

关 键 词：离子束注入 硅酸盐细菌 选育 

分 类 号：Q93[生物学—微生物学]