人旋毛虫病血清与诊断抗原的检测方法的比较研究  

COMPARATIVE STUDIES ON SERODIAGNOSTIC METHODS AND DIAGNOSTIC ANTIGENS FOR HUMAN TRICHINOSIS

作　　者：李燎[1] 雷莉[2] 闫和平[2] 冯瑞元[2] 

机构地区：[1]泸州医学院附属医院皮肤科,646000 [2]华西医科大学寄生虫学教研室

出　　处：《泸州医学院学报》1996年第3期178-181,共4页Journal of Luzhou Medical College

基　　金：卫生部基金

摘　　要：以旋毛虫肌肉期幼虫的分泌排泄抗原（ES）和纯化ES中46～58KD抗原（P－ES）为诊断抗原，用Dot－ELISA同时检测34份旋毛虫病人血清，阳性检出率分别为91．18％和94．12％（P＞0．05）．而100份正常人血清均为阴性；检测其它4种蠕虫病人血清62份，交叉反应率分别为29．3％和0％，P－ES的特异性明显优于ES（P＜0．05）。另外，以P-ES为珍断抗原，比较Dot-ELISA和间接ELISA用于诊断旋毛虫病人的检测效果，实验表明：Dot-ELISA不仅有间接ELISA相似的敏感性和特异性，而且简便、快速、经济，更适合于推广应用。In the first place, the diagnostic value of excretorysecretory antigen (ES) of Trichinella spiralis muscle larvae and its purified 46-58 KD antigen (P-ES) was tested by Dot-ELISA. The positive rates of 34 sera from confirmed trichinosis patients using ES and P-ES as diagnostic antigen were 91. 18% and 94. 12% respectively (P>0. 05). The false positive reactions of 100 normal human sera were not found. in the study. The cross reaction rates of 62 sera of patients with other helminthiasis were 29. 03% and 0% respectively, the specifity of P-ES was obviouly higher than that of ES (P<0. 05) Next, comparision of Dot-ELISA and indirect ELISA using P-ES as diagnostic antigen for detecting human trichinosis was made. The results indicated that Dot-ELISA is not only as sensitive and specific as indirect ELISA but more rapid, simple, economical and feasible in field work or in rural area.

关 键 词：人 旋毛线虫病 诊断 抗原 血清 

分 类 号：R532.140.4[医药卫生—内科学] R446.62[医药卫生—临床医学]