3种鱼类致病菌粗脂多糖(LPS)对斑点叉尾(鱼回)的免疫原性  

Immunogenicity of Crude Lipopolysaccharide of Three Pathogenic Bacteria to Channal Catfish,Ictalurus punctatus

作　　者：刘勇[1] 汪成竹[1] 陈昌福[1] 

机构地区：[1]华中农业大学水产学院,湖北武汉430070

出　　处：《长江大学学报（自科版）（中旬）》2006年第4期170-173,182,共5页Journal of Yangtze University(Nature Science Edition)

基　　金：国家自然科学基金资助项目(30271032)

摘　　要：用温酚法分别从柱状嗜纤维菌(Cytophaga columnaris)、嗜水气单胞菌(Aeromonas hydrophila)和叉尾鮰爱德华菌(Edwardsiella ictaluri)3种鱼类致病菌中提取粗脂多糖(LPS)作为免疫原,分别接种斑点叉尾鮰(Ictalurus punctalus Rafinsque)后,通过测定受免鱼的交叉凝集抗体效价、头肾和血液中吞噬细胞的吞噬活性以及对A.hydrophila活菌细胞内的杀菌活性,探讨了3种致病菌的粗LPS对斑点叉尾鮰的免疫原性。结果表明,从3种致病菌中提取的粗LPS对斑点叉尾鮰均具有较强的免疫原性,受免鱼的血清中存在对3种致病菌的交叉凝集抗体,与对照鱼相比,头肾和血液中吞噬细胞对吞噬原的吞噬活性和对A.hydrophila活菌杀菌活性明显上升。说明在3种供试菌的粗LPS上不同程度地存在3种致病菌的共同保护性抗原。Channal catfish(Ictalurus punctatus Rafinsque)were immunized with crude lipopolysaccharide(LPS) extracted from Cytophaga columnaris,Aeromonas hydrophila and Edwardsiella(ictaluri).The immunogenicity of crude lipopolysaccharide to channal catfish were compared by examining the agglutinating antibody titers,cross-agglutinating antibody titers of serum,the phagocytic activity and bactericidal activity of phagocytes in the blood and head kidney.The results showed that the agglutinating antibody titers and cross...

关 键 词：柱状嗜纤维菌(Cytophaga columnaris) 嗜水气单胞菌(Aeromonns hydrophiila) 叉尾鮰爱德华菌(Edwardsiella ictaluri) 粗脂多糖 斑点叉尾鮰(Ictalurus punctatus Rafinsque) 免疫原性 

分 类 号：S942.5[农业科学—水产养殖] Q959.468[农业科学—水产科学]