小肠RNA对受^(60)Co γ射线照射后小鼠肠粘膜屏障的保护作用  

Rotective effects of intestinal RNA on intestinal mucosal barrier in mice after abdominal γ-irradiation

作　　者：苏晓明[1] 曾桂英[1] 任东青[1] 田锐锋[2] 周晓光[1] 赵涛[1] 王爽[3] 杨家骥[3] 徐修礼[4] 

机构地区：[1]第四军医大学军事预防医学系放射医学教研室,西安710032 [2]第四军医大学校直干休所,西安710032 [3]第四军医大学基础部电镜中心,西安710032 [4]第四军医大学西京医院检验科,西安710033

出　　处：《辐射研究与辐射工艺学报》2006年第6期356-361,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30370440)资助

摘　　要：以BALB/c小鼠为研究对象,用60Coγ射线进行11.5Gy的腹部一次照射,照后1—3h采用局部肠腔扩张注入法给小鼠空肠肠腔内注入正常大鼠小肠核糖核酸(Ribonucleicacid,RNA),照后1、3、5d取全小肠,制备肠粘液,测定免疫球蛋白分泌型免疫球蛋白A(SecretedimmunoglobulinA,sIgA)含量;取空肠段,观察肠绒毛形态变化;取血,测定内毒素含量;取肠系膜淋巴结,测定细菌移位率。探讨小肠RNA对γ射线照射后小鼠肠粘膜屏障的保护作用。结果表明,小肠RNA可明显提高受照后小鼠肠粘液中免疫球蛋白sIgA的含量(p<0.01),减轻肠绒毛的萎缩及塌陷,改善肠粘膜形态结构,降低肠系膜淋巴结细菌移位率和血中内毒素含量。以上结果显示,小肠RNA对γ线照射后的小鼠肠粘膜屏障有明显的保护作用,能抑制肠道细菌移位和肠内内毒素的吸收。The work was aimed at exploring protective effects of intestinal RNA on intestinal mucosal barrier in mice after abdominal γ-irradiation. The BALB/c male mice were abdominally irradiated with 11.50Gy ^60Co γ-ray in 1-3h, and then they were injected intestinal RNA from normal on jejunum. On 1, 3 and 5d after irradiation, the mice were sacrified after anesthesia for determining sIgA in small intestinal mucilage, endotoxin in blood and bacterial metathetic rate, and observing morphological changes of jejunal villus. The result showed that intestinal RNA can decrease MLN bacterial metathetic rate and the level of endotoxin in blood, and increase sIgA in small intestinal mucilage （p〈0.01）. In addition, it can improve intestinal mucosal morphology and reduce atrophy and collapse of jejunal villus. In conclusion, Intestinal RNA can improve intestinal mucosal barrier and inhibit intestinal bacterial translocation and absorption of intestinal endotoxin in irradiated mice.

关 键 词：小肠核糖核酸 Γ射线 分泌型免疫球蛋白A 内毒素 细菌移位 

分 类 号：R818.05[医药卫生—放射医学] Q691.5[医药卫生—临床医学]