WORT增强γ射线对SHG44细胞周期及凋亡的影响  

Enhancement of wortmannin on ^(60)Co γ-irradiation effect of cell cycle procession and apoptosis induction in SHG-44 cells

作　　者：薛景[1] 刘芬菊[1] 俞家华[1] 李冰燕[1] 杨学琴[1] 孙志强[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院

出　　处：《辐射研究与辐射工艺学报》2006年第6期373-377,共5页Journal of Radiation Research and Radiation Processing

基　　金：苏州大学医学发展基金重点项目(EE126031);江苏省放射医学重中之重学科(FY2004)资助

摘　　要：研究渥曼青霉素(Wortmannin,WORT)对人脑胶质瘤细胞辐射增敏作用及诱导细胞凋亡。用克隆法、流式细胞术和激光共聚焦显微镜扫描技术检测细胞克隆形成、细胞周期和凋亡以及凋亡的形态学特征。结果表明:WORT能抑制细胞克隆形成,和60Coγ射线联用抑制作用增强,表现出协同作用。WORT和60Coγ射线联用能引起SHG-44细胞G1期阻滞,WORT能增强60Coγ射线诱导的细胞凋亡,凋亡细胞核固缩和核碎裂,可见凋亡小体。以上结果表明,WORT可能通过诱导细胞G1期阻滞和凋亡途径来增加细胞的辐射敏感性。To study radiosensitizing effect and apoptosis induction of wortmannin on human glioma cells, colony formation assay, flow cytometry, laser confocal microscope were used to investigate the inhibition effect on colony formation, apoptosis and cell cycle procession. Experiment results suggested that wortmannin could inhibit colony formation in SHG-44 cells, and the combination of wortmannin with ^60Co γ-irradiation showed a synergistic action. Wortmannin could also induce G1 arrest, apoptosis, and morphologyical changes such as karyopyknosis, nuclear-fragmentation and apoptotic body were observed. The above results revealed that wortmannin can sensitize SHG-44 cells to gama radiation, which might be related with G1 arrest and apoptosis pathway.

关 键 词：渥曼青霉素 脑胶质瘤细胞 辐射增敏 凋亡 

分 类 号：R811.5[医药卫生—放射医学] R730.5[医药卫生—临床医学]