人脐血来源树突状细胞体外扩增及诱导特异性抗宫颈癌免疫应答  

In Vitro Generation of Dendritic Cells from Cord Blood and Induction of Specific Antitumor Immunity in Cervical Carcinoma Cells

作　　者：王华丽[1] 孔北华[2] 张建华[3] 张彩[3] 

机构地区：[1]山东大学第二医院,山东济南250033 [2]山东大学齐鲁医院,山东济南250012 [3]山东省医学科学院基础医学研究所,山东济南250062

出　　处：《实用妇产科杂志》2006年第12期722-724,共3页Journal of Practical Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(30271361);山东省卫生厅科技计划2005-14(青年基金)

摘　　要：目的:研究脐血来源树突状细胞(DC)体外扩增及诱导特异性抗宫颈癌细胞的免疫效应。方法:自人脐血分离单核细胞诱导DC,制备宫颈癌细胞系CaSki冻融物作为抗原负载DC。ELISA法检测成熟DC上清中IL-12的含量,混合淋巴细胞反应(MLR)测定DC体外刺激T细胞增殖的能力,MTT法检测抗原负载DC激活的CTL对宫颈癌细胞CaSki和HeLa的杀伤作用。结果:与未经抗原负载的DC相比,抗原负载的DC刺激同种异体T细胞增殖和IL-12分泌的能力均有提高(P<0·05),激活的CTL对CaSki细胞有特异性杀伤,而此CTL对HeLa细胞无特异性杀伤。结论:宫颈癌细胞CaSki冻融抗原负载DC激活的CTL可诱导出特异性杀伤宫颈癌细胞的免疫效应。Objectlve;To investigate the ability of generation of dendritic cell （DC） from precursor in cord blood and inducing specific antitumor immune response. Methods： Monocytes were isolated from cord blood and cultured to differentiate into DC induced by cytokines. DCs were loaded with freeze-thaw antigen of CaSki cells. IL-12 production in DC suspension was detected using ELISA method,and DC function was evaluated their ability to induce proliferation of allogenaic T cells by mixed lymphocyte reaction （MLR） assay. The cytotoxicity to CaSki cells and Hela cells of CTL activated by dendritic cells loaded with antigen was detected by MTT method. Results：Antigen-loaded DC had strong ability of inducing proliferation of allogeneic T cells and more secretion of IL-12 than that of DC without loading antigen. And activated CTL had specific antitumor effect to CaSki cells, not to HeLa. Conclusions： CTL activated by DC loaded with freeze-thaw antigen of CaSki cells could induce most potent specific cytotoxicity to cervical carcinoma cells in vitro.

关 键 词：树突状细胞 宫颈肿瘤 冻融抗原 细胞毒性T淋巴细胞 

分 类 号：R711.74[医药卫生—妇产科学]