9．1C3单克隆抗体可溶性单链抗体基因的表达及其初步鉴定  

Expression and Preliminary Identification of the Soluble Single -chain Fv of 9.1C3 McAb

作　　者：夏海滨[1] 金伯泉[1] 马文煜[1] 田方[1] 

机构地区：[1]第四军医大学免疫学教研室,第四军医大学微生物学教研室

出　　处：《细胞与分子免疫学杂志》1996年第4期7-10,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金："九五"全军医药卫生科研基金

摘　　要：用噬菌粒表达载体ｐＣＡＮＴＡＢ５Ｅ构建含９．１Ｃ３单链抗体基因片段的重组表达栽体，经ＩＰＴＧ诱导后可表达一分子量约为３０ｋＤａ的可溶性融合蛋白。体外生物学实验表明，诱导表达的９．１Ｃ３单链抗体，可阻断ＮＫ细胞对靶细胞Ｋ５６２的杀伤。此结果为临床应用９．１Ｃ３单链抗体治疗自身免疫性疾病和预防移植排异反应提供了基础。The rccombinant expression vector containing 9.1C3 ScFv gene was constructed by using phagemid expression vector pCANTAB5E, The expression of a soluble fusion protein with molecular weight of 30 kDa was induced by IPTG. The biological activity of expression products of 9. 1C3 ScFv gene was determined by 51Cr rel ease assay in vitro. The results showed that the induced 9. 1C3 ScFv could block the cytotoxicity medicated by NK cells. The success of 9.1 C3ScFv gene expression previde the possibility of the application of 9.1C3 ScFv in treating some autoimmunne diseases and preventing allograft rejection.

关 键 词：单链抗体 NK细胞 单克隆抗体 基因 9.1C3 

分 类 号：R392.11[医药卫生—免疫学] R394[医药卫生—基础医学]