TLSF_（JM）对DNFB诱导的小鼠接触性皮炎和同种异体皮片移植排斥反应的抑制作用  

The Suppressive Effect of TLSF_JM on DNFB-induced Contact Sensitivity and the Rejection to the Skin Allografts in Mice

作　　者：申立中[1] 金伯泉[1] 夏海滨[1] 朱勇[1] 刘雪松[1] 

机构地区：[1]第四军医大学免疫学教研室

出　　处：《细胞与分子免疫学杂志》1996年第4期27-31,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金

摘　　要：本文首次对急性Ｔ淋巴细胞白血病ＪＭ细胞系所分泌的抑制因子（ＴＬＳＦＪＭ），在体内的免疫调节作用进行了研究。结果表明：（１）体内应用ＴＬＳＦＪＭ可明显抑制由ＤＮＦＢ所诱导的小鼠接触性皮炎，降低局部针对变应原的特异性炎症反应；（２）ＴＬＳＦＪＭ能有效地延长移植在Ｃ５７ＢＬ／６小鼠上的ＢＡＬＢ／ｃ小鼠皮片的存活时间，并降低体外混合淋巴细胞反应中受体淋巴细胞对供体同种异体抗原刺激的再次增殖反应。The in vivo immunosuppressive effect of TLSFJM on DNFB-induced contact sensitivity and skin transplantation was investigated. Affer i. p. twice injection of TLSFJM, in BALB/c mice sensitized and then challenged with DNFB, the increment of the challenged left ear decreased markedly as compared with RPMI 1640 injected mice. However, in only vehicle-sensitized and then DNFB-challenged mice, there was no diference between TLSFJM and RPMI 1640 treatment. Adult C57BL/6 mice were given skin allografts from BALB/c mice. In recipient mice, the treatment with i. p. injection of TLSFJM after transplantation resulted in significantly much longer survival time of the skin grafts, with median survival time(MST) of 23. 4 days, than the group treated with RPMI 1640 (MST=10.6 days). The proliferative reaction of splenocytes from TLSFJM treated mice (H-2b)in response to stimulation by the donor's splenocytes (H-2d)decreased 38. 6% in comparsion to RPMI 1640 treated mice.

关 键 词：抑制因子 接触性皮炎 移植排斥反应 免疫抑制 

分 类 号：R622.103[医药卫生—整形外科] R758.220.2[医药卫生—外科学]