伊凡思蓝检测糖尿病大鼠血-视网膜屏障通透性改变的方法探讨  

Evans Blue Technique for Quantitating Blood_retinal Barrier Breakdown

作　　者：刘乃慧[1] 王康[1] 李新民[1] 

机构地区：[1]首都医科大学附属北京友谊医院实验中心,北京100050

出　　处：《实验动物科学与管理》2006年第4期51-52,58,共3页Laboratory Animal Science & Administration

摘　　要：目的探讨伊凡思蓝(EB)作为检测早期糖尿病大鼠血_视网膜屏障通透性改变的方法的可行性。方法选用Brown_Norway大鼠,进行糖尿病造模1周后,模型组与对照组分别血管内注射EB,检测视网膜中含量。结果对照组和糖尿病组视网膜内EB含量分别为(13.22±3.59)ng/mg(n=12)和(23.41±4.47)ng/mg(n=12),结果差异有显著性(P<0.05)。结论静注后检测视网膜内EB含量的方法,可以比较精确地发现早期糖尿病大鼠视网膜病变中血_视网膜通透性的改变。Objective To investigate whether Evans blue can be adapted as a method for quantitating blood-retinal barrier breakdown. Methods The content of Evans blue in the retina of normal and 1 week diabetic Brown-Norway rats were detected by absorption value after intravenous injection. Results Retinal Evans blue leakage of eyes from 1 week diabetic rats was（23.41 ± 4.47）ng/mg （n = 12）, which was much higher than（13.22 ± 3.59）ng/mg （n = 12） from normal ones （ P 〈 0.05 ）. Conclusions The Evans blue technique can be modified to a sensitive and quantitative method for measuring blood-retinal barrier breakdown.

关 键 词：糖尿病 伊凡思蓝 视网膜 大鼠 

分 类 号：Q95-331[生物学—动物学]