山豆根碱对大鼠血小板摄取^（45）Ca^（2＋）的影响  

Effect of dauricine on^(45)Ca^(2+)uptake into platelets in rats

作　　者：刘俊田[1] 邓秀玲[1] 邱培伦[1] 

机构地区：[1]西安医科大学药理学教研室

出　　处：《中国药理学通报》1996年第5期412-414,共3页Chinese Pharmacological Bulletin

摘　　要：用４５Ｃａ２＋摄入法测定了大鼠血小板摄取细胞外Ｃ２＋，观察山豆根碱对血小板摄取Ｃａ２＋的影响、结果表明，静息血小板可迅速摄取细胞外Ｃａ２＋，呈时间和浓度依赖性；ＡＤＰ促进血小板摄取Ｃａ２＋；山豆根碱以浓度依赖方式抑制静息的和ＡＤＰ诱导的血小板摄取细胞外Ｃａ２＋。这提示山豆根碱抑制血小板聚集的作用机制之一与阻止细胞外Ｃａ２＋内流入血小板，降低血小板胞浆内Ｃａ２＋浓度有关。The uptake of extracellular Ca2+ into rat Platelets was determined with 45Ca2+ uptake method and effects of dauricine on the uptake were studied.The results indicated that 45Ca2+ was rapidly taken up into resting platelets and 45Ca2+ uptake increased after stimulation of platelets with ADP;dauricine significantly inhibited 45Ca2+ uptake into resting and ADP-stimulated platelets in the concentration-dependent way,IC50 were 0.63 and0.29 g·L-1 respectively;and that verapamil inhibited only ADP-induced 45Ca2+ uptake into plateltes and had no effect on 45Ca2+ uptake into resting platelets.These results suggest that one of the action mechanisms of antiaggregation by dauricine is related to the inhibition of extracellular Ca2+ influx into platelets.

关 键 词：山豆根碱 血小板 钙离子 药理学 

分 类 号：R285.5[医药卫生—中药学] R282.710.5[医药卫生—中医学]