血管紧张素Ⅱ对培养乳鼠非心肌细胞DNA及蛋白合成的影响  

Effects of angiotensin Ⅱ on DNA and protein synthesis in cultured cardiac nonmyocytes of neonatal rats

作　　者：王洪新[1] 陶亮[1] 饶曼人[1] 

机构地区：[1]南京医科大学心血管药理研究室,锦州医学院药理教研室

出　　处：《中国药理学通报》1996年第5期417-420,共4页Chinese Pharmacological Bulletin

摘　　要：ＡｕｇⅡ作为一种独立于血液动力学及神经体液因子，对哺乳动物心脏细胞的直接作用尚未完全清楚。本实验中，我们观察了ＡｕｇⅡ对培养心脏的非心肌细胞的作用，结果表明：在培养的非心肌细胞，ＡｕｇⅡ引起蛋白合成、ＤＮＡ合成及细胞数目的明显增加，但不影响细胞的体积。ＡｕｇⅡ在非心肌细胞诱导的ＤＮＡ合成受ＡｕｇⅡ受体拮抗剂［Ｓａｒ１，Ｖａｌ５，Ａｌａ８］－ＡｕｇⅡ完全抑制、这些结果提示：（１）ＡｕｇⅡ诱导心脏非心肌细胞的增殖；（２）ＡｎｇⅡ的上述作用通过ＡＴ１受体亚型调控。Increasing evidence suggests that angiotensin Ⅱ(AugⅡ)may act as a growth factor for the heart.However,direct effects of Aug Ⅱon cardiac nonmyocytes-independent of secondary hemodynamic and neurohumoral effects have not been well characterized.In this experiment, we observed effects of Aug Ⅱon cultured cardiac nonmyocytes.The results showed that Ang Ⅱ1,10,100,1000nmol·L-1 induced increases in protein synthesis,DNA synthesis and cell number without changing cell volume in nonmyocytes.The Aug Ⅱ-induced DNA synthesis in nonmyocytes was fully blocked by[Sar1,Val5,Ala8]-Aug Ⅱ which suggested that:(1) Aug Ⅱcaused hyperplasla of cardiac nonmyocytes(2)The effects of AugⅡ examined were mediated primarily by the AT1 receptor subtype.

关 键 词：血管紧张素 蛋白 心肌肥厚 受体DNA 

分 类 号：R972[医药卫生—药品] R966[医药卫生—药学]