HgCl_2中毒大鼠脊髓胶质纤维酸性蛋白表达上调  

GFAP Level is Increased in Spinal Cord of HgCl_2 Poisoning Rat

作　　者：周永田[1] 徐旭东[1] 谢林[1] 戴平[1] 马娟[1] 史春霞[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院,济南250002

出　　处：《职业卫生与应急救援》2006年第4期172-174,共3页Occupational Health and Emergency Rescue

摘　　要：目的观察HgCl2对大鼠脊髓星形胶质细胞的影响。方法SD大鼠30只,雌雄各半,体重160￣200g。随机分为3组,每组10只(5♀,5♂)。①大剂量组,按17mg/kg(1/4LD50)经口灌胃HgCl2溶液,每天1次。②小剂量组,按8.5mg/kg(1/8LD50)经口灌胃HgCl2溶液,每天1次。③对照组,经口灌胃生理盐水2ml,每天1次。三组均连续灌胃(20±2)d,染汞组制成亚急性汞中毒模型后,各组均处死5只雄鼠,留5只雌鼠作药物干预实验。用二巯丙磺钠(DMPS)按28mg/kg腹腔注射驱汞2疗程,在此基础上,大剂量组加用己酮可可碱(POF),小剂量组仅用DMPS。实验结束后处死全部动物,灌注固定取出脊髓L5-6节并制成病理切片。用免疫组化SABC法测定脊髓胶质纤维酸性蛋白(GFAP)。结果两染汞组GFAP平均灰度均显著低于对照组,阳性细胞率均显著高于对照组。DMPS+POF显著降低GFAP阳性细胞率,提高平均灰度值,单纯应用DMPS无此作用。结论亚急性HgCl2中毒大鼠脊髓GFAP表达上调,POF能够有效抑制脊髓星形胶质细胞激活。Objective To observe the effect of HgCl2 on the astrocyte in spinal cord of rats. Methods Thirty SD rats （15 ♀ ,15 ♂, weighting 160 ～ 200 g） were randomized into 3 groups, Group A and group B received HgCl2 at a dose of 17 mg/kg （1/4 LD50） , 8.5 mg/kg （1/8 LD50） respectively by garage daily and Group C received 0.9% saline 2 ral by garage daily. The sub-acute mercury poisoning rat model were made within （20±2） days. Then male rats in each group were dissected and the chelator （DMPS） was injected into the abdomen of female rats of group A and B at a dose of 28 mg/kg for 2 periods. Group A received DMPS ＋POF （pentoxifyUine） and group B received DMPS only. The rats were all dissected and the spinal cord （L5-6） were gotten after the trial finished. Then the pathological section of spinal cord was prepared and GFAP was detected. Results The average gray scale in group A and group B are all less and the ratio of positive cell numbers were all larger significantly compared with the control group. The POF, not DMPS, depress the activation of astrocyte significantly. Conclusion Sub-acute poisoning of HgCl2 could increase GFAP level significantly in spinal cord, and POF depresses the activation of astrocyte.

关 键 词：汞中毒 神经病理性疼痛 脊髓 星形胶质细胞 胶质纤维酸性蛋白 

分 类 号：R135.13[医药卫生—劳动卫生]