青霉素诱导小鼠产生特异性非IgE抗体应答  

Benzylpenicillin induced specific non IgE antibody response in mice

作　　者：张蕾[1] 王永铭[1] 陈斌艳[1] 沈刚[1] 

机构地区：[1]上海医科大学药学院药理教研室 儿科医院

出　　处：《中国药理学报》1996年第6期531-534,共4页Acta Pharmacologica Sinica

摘　　要：青霉素诱导小鼠产生特异性非ＩｇＥ抗体应答张蕾，王永铭，陈斌艳，沈刚１（上海医科大学药学院药理教研室；１儿科医院，上海２０００３２，中国）关键词青霉素类；免疫球蛋白Ｍ；免疫球蛋白Ｇ；免疫球蛋白Ａ；酶联免疫吸附试验；半抗原；交叉反应；药物过敏目的：研究青...IM: To study whether or not the specific non IgE antibody response in mice can be induced by benzylpenicillin in vivo . METHODS: Antibody response and antigenic cross reactions were determined by enzyme linked immunosorbent assay (ELISA). Antigen molecules recognized by antibodies were tested by hapten inhibition assay. RESULTS: During d 1-d 50 after immunization, positive % of specific IgM, IgG, and IgA to benzylpenicillin were 100 % , 50 %-100 % , and 17 %-100 % , respectively. IgM and IgG to benzylpenicillin also recognized ampicillin and piperacillin. The positive % of IgM and IgG to ampicillin were 23 %-100 % and 50 %-100 % , to piperacillin 43 %-100 % and 50 %-100 % , respectively. Aged benzylpenicillin showed an inhibitory effect on specific antibodies in a dose dependent manner, and inhibitory % of specific IgM and IgG were 29 %-87 % and 29 %-71 % . However, freshly prepared benzylpenicillin had no effect. CONCLUSION: Specific non IgE antibody response was successfully induced by benzyl￣ penicillin in mice, in which the isotypes were mainly IgM and then IgG and IgA. Antibodies recognized degraded products, not benzylpenicillin molecule itself. Antigenic cross reactions occurred between benzylpenicillin, ampicillin, and piperacillin. Isotypes of antibodies responsible for cross reactions were mainly IgG and then IgM.

关 键 词：青霉素 免疫球蛋白 药物过敏 ELISA 抗体 

分 类 号：R967[医药卫生—药理学] R978.11[医药卫生—药学]