戈米辛J对肝线粒体膜和培养心肌细胞的抗脂质过氧化作用  

Anti lipid peroxidation of gomisin J on liver mitochondria and cultured myocardial cells 1

作　　者：彭红丽[1] 陈道峰[1] 蓝洪祥 张雪梅[1] 顾峥[1] 江明华[1] 

机构地区：[1]上海医科大学药学院药理教研室 生药教研室

出　　处：《中国药理学报》1996年第6期538-541,共4页Acta Pharmacologica Sinica

基　　金：the;National;Natural;Science;Foundation;of;China;and;Science;and;Technology;Commision;of;Shanghai;Municipality

摘　　要：戈米辛Ｊ对肝线粒体膜和培养心肌细胞的抗脂质过氧化作用１彭红丽，陈道峰２，蓝洪祥，张雪梅，顾峥，江明华（上海医科大学药学院药理教研室；２生药教研室，上海２０００３２，中国）关键词脂质过氧化；戈米辛Ｊ；木脂素；培养的细胞；肝线粒体；心肌目的：研究来自内南...IM: To study the influences of gomisin J on lipid peroxidation and calcium paradox. METHODS: Using two in vitro models of rat liver mitochondria membrane lipid peroxidation (LPO) and cultured myocardial cells. RESULTS: Gomisin J inhibited Fe 2+ /ascorbic acid and ADP/NADPH induced LPO with IC 50 ( 95 % confidence limits) 5 5 (4 5-6 7) and 4 7 ( 2 8-7.8) μmol·L -1 , respectively, when cultured myocardial cells preincubated with Ca 2+ free medium for 2 min were incubated with normal medium containing Ca 2+ , a marked increase of malondialdehyde (MDA) formation occurred and gomisin J 10 μmol·L -1 protected myocardial cells through decreasing MDA formation. CONCLUSION: Gomisin J inhibits LPO in rat liver mitochondria and protects cultured myocardial cells from being injuried by calcium paradox.

关 键 词：过氧化脂质 戈米辛J 木脂素 肝线粒体 心肌 

分 类 号：R972[医药卫生—药品] R965.1[医药卫生—药学]