米邦塔食用仙人掌组培快繁技术研究  

A Study on the Rapid Propagation Method of Opuntia vilpa alta Haw.Plant Tissue Culture in Vitro Plant Tissuecultuk

作　　者：陈芳[1] 李桂芳[1] 魏鹏[1] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003

出　　处：《石河子大学学报（自然科学版）》2006年第5期596-598,共3页Journal of Shihezi University(Natural Science)

摘　　要：探索了米邦塔食用仙人掌组培快繁技术,得出不定芽诱导使用培养基MS+6-BA 3.0mg/L+NAA 0.1mg/L,外植体分化率达77.3%;继代增殖使用培养基MS+6-BA 1mg/L+KT 0.5mg/L+NAA 0.3mg/L,试管苗平均增殖倍数达10.19,结合反复利用原外植体的方法,增殖效率可进一步提高;生根培养基使用1/2 MS+NAA 0.1mg/L+IBA 0.1mg/L,生根率达100%,且根系发育优良;驯化及试管苗入地后,以保湿为主要管理措施,可保证移栽成活率在95%左右。The results showed explants of differentiation rate attained to 77.3% with medium MS ＋ 6-BA 3.0mg/L ＋ NAA 0. 1mg/L in bud induction; the tube seedling multiplication attained 10.19 in subculture with medium MS ＋ 6-BA 1 mg/L ＋ KT 0.5mg/L ＋ NAA 0.3mg/L. The multiplication efficiency migh t further enhance ff explants were used repeatedly. Rooting rote attained to 100% with medium 1/2 MS ＋ NAA 0. 1mg/L ＋ IBA 0. 1mg/L, and growth of the rooting seedling was fine; After sunlight domesticating, the seedling transplanting survival rate attained to about 95%.

关 键 词：食用仙人掌 组培快繁 增殖效率 

分 类 号：S644.9[农业科学—蔬菜学]