高效液相色谱法测定脑灵口服液中人参皂苷Rg_1的含量  

Determination of Ginsenoside Rg_1 in Naoling Oral Liquid by HPLC

作　　者：张莉[1] 王继生[1] 胡尚久[1] 蒋渝[1] 龚晶淼[1] 

机构地区：[1]四川绵阳市第三人民医院,绵阳市621000

出　　处：《中国药房》2006年第24期1902-1903,共2页China Pharmacy

摘　　要：目的:建立以高效液相色谱法测定脑灵口服液中人参皂苷Rg1含量的方法。方法:色谱柱为HypersilBDS-C18,流动相为甲醇-水(49∶51),流速为1.0ml/min,检测波长为203nm,柱温为30℃,进样量为20μl。结果:人参皂苷Rg1进样量在0.2μg～8.0μg范围内与峰面积积分值线性关系良好(r=0.9996),平均加样回收率为101.25%(RSD=1.54%)。结论:本方法简便、准确、重现性好,可用于本品的质量控制。OBJECTIVE： To establish an HPLC method for the determination of Ginsenoside Rg1 in Naoling oral liquid METHODS： The assaying was conducted on a Hypersil BDS- C18 with methol- water（49 ： 51） as mobile phase.The detective wavelength was set at 203nm;the column temperature was 30℃ ;the flow rate was 1.0ml/min and the sample size was 20μl.RESULTS： The linear concentration range of Ginsenoside Rgl was 0.2μg-8.0μg（r = 0.9 996）; the average recovery was 101.25% （RSD- 1.54% ）.CONCLUSION：The method issimple, accurate and reproducible;and it can be used for the quality control of Ginsenoside Rg1.

关 键 词：高效液相色谱法 脑灵口服液 人参皂苷RG1 含量 

分 类 号：R927.2[医药卫生—药学]