Transforming Growth Factor-β Expression Induced by Rhinovirus Infection in Respiratory Epithelial Cells  

作　　者：Amrita DOSANJH 

机构地区：[1]Department of Molecular and Experimental Medicine,the Scripps Research Institute,La Jolla 92037,USA

出　　处：《Acta Biochimica et Biophysica Sinica》2006年第12期911-914,共4页生物化学与生物物理学报（英文版）

基　　金：This work was supported by a grant from the NIH/NIAID fund

摘　　要：Rhinovirus infection of the lower airways is now a recognized disease, associated with bronchiolitis and asthma. The bronchial epithelial cells are the host cells when rhinovirus infection occurs in the airway. It was hypothesized that a pro-fibrotic growth factor response may occur in these infected cells, leading to production of a key transforming growth factor, TGF-β-1. Bronchial epithelial cells were inoculated with human rhinovirus and compared at day 1, 3 and 5 to control non-infected cells. Cell culture supernatant fluid and cellular RNA were isolated. The amount of released TGF-β protein was measured by enzyme-linked immunosorbent assay （ELISA）. Expression of TGF-β at the level of transcription was measured by polymerase chain reaction （PCR） and gel electrophoresis. The results show that at all time points studied, TGF-β production is greater in the infected cells, as demonstrated by ELISA （P〈0.05） and by semiquantitative PCR analysis. It was concluded that bronchial epithelial cells infected with common cold virus and rhinovirus, showed higher levels of TGF-β. The production of TGF-β may be indicative of a normal repair mechanism to counter inflammation, or in the setting of persistent asthma, could potentially lead to increased fibrosis and collagen deposition.Rhinovirus infection of the lower airways is now a recognized disease, associated with bronchiolitis and asthma. The bronchial epithelial cells are the host cells when rhinovirus infection occurs in the airway. It was hypothesized that a pro-fibrotic growth factor response may occur in these infected cells, leading to production of a key transforming growth factor, TGF-β-1. Bronchial epithelial cells were inoculated with human rhinovirus and compared at day 1, 3 and 5 to control non-infected cells. Cell culture supernatant fluid and cellular RNA were isolated. The amount of released TGF-β protein was measured by enzyme-linked immunosorbent assay （ELISA）. Expression of TGF-β at the level of transcription was measured by polymerase chain reaction （PCR） and gel electrophoresis. The results show that at all time points studied, TGF-β production is greater in the infected cells, as demonstrated by ELISA （P〈0.05） and by semiquantitative PCR analysis. It was concluded that bronchial epithelial cells infected with common cold virus and rhinovirus, showed higher levels of TGF-β. The production of TGF-β may be indicative of a normal repair mechanism to counter inflammation, or in the setting of persistent asthma, could potentially lead to increased fibrosis and collagen deposition.

关 键 词：RHINOVIRUS TGF-β BRONCHIAL ASTHMA common cold 

分 类 号：R373[医药卫生—病原生物学]