银杏叶总黄酮介导的豚鼠腹腔神经节细胞的去极化  

Total flavone ginkgo-mediated depolarization in the neurons of guinea pig celiac ganglia in vitro

作　　者：胡金兰[1] 张玲[2] 孔淼[1] 柯道平[1] 黄九龙[1] 李忠稳[1] 马嵘[1] 孔德虎[1] 

机构地区：[1]安徽医科大学生理学教研室神经生理研究室,合肥230032 [2]华东师范大学上海脑功能基因组学研究所,上海200062

出　　处：《安徽医科大学学报》2006年第6期659-663,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学基金资助项目(编号:2006KJ362B;2005KJ263)

摘　　要：目的观察并分析银杏叶总黄酮(TFG)对豚鼠离体腹腔神经节(CG)细胞膜电位的影响。方法应用细胞内生物电记录技术和离体神经节灌流给药方法。结果TFG可使45·8%的CG细胞去极化,幅度和时程分别为(7·9±0·7)mV和(245·9±29·2)s,且表现剂量依赖性;10%超极化;另有10%出现先超极化后去极化的双相反应;还有34%几乎不发生反应。多数细胞的去极化可被低钙/高镁Krebs液部分阻断,但不受胆碱和肾上腺素受体阻断剂的影响。在TFG诱发的CG细胞去极化的同时伴膜电阻减小(47·2%)、增大(21·8%)或不变(30·9%);无钠或无钙的Krebs液可部分阻断CG细胞去极化,而四乙基铵则使细胞去极化增强。结论TFG可诱发豚鼠CG大多数细胞的去极化,其机制可能与Na+、Ca2+内流增强和K+外流减弱有关。Objective To investigate and analyze the effect of the total flavone ginkgo （TFG） on membrane potential of the neurons in celiac ganglia （CG） of guinea-pig in vitro. Methods Intracellular microelectrode recording and administration of superfusion were used. Results Superfusion of celiac ganglia with TFG evoked a concentration-dependent membrane depolarization in 45.8% of the neurons, amplitude and duration are 7.9 mV ± 0. 7 mV and 245.9 s ±29.2 s respectively and 10% demonstrated hyperpolarization and 10% biphasic response which consisted of an initial hyperpolarization phase and a followed depolarization, also 34% no response to TFG. The depolarization of most neurons could be blocked partly by cholinoceptor （atropine and hexamethonium） and adrenoceptor antagonist （phentolamin and propranolol） ; Among the depolarization neurons, the membrane resistance （Rm） decreased in 47. 2% increased in 21.8% and unchanged in 30. 9% ; The depolarization can be blocked partly by Na^＋ and Ca^2＋ free Krebs solution and depolarization increased by traethylammonium （TEA）. Conclusion TFG can evoke the depolarization of most CG neurons, and the mechanism probably is associated with the increasing in- flux of Na^＋ and Ca^2＋ and the decreasing outflow of K^＋.

关 键 词：膜电位/药物作用 神经节交感 豚鼠 

分 类 号：R282.71[医药卫生—中药学] R329.25[医药卫生—中医学]