白细胞介素1对椎间盘细胞软骨特异性基因Sox9 mRNA的调节作用  

The regulation effect of interleukin-1 on expression of cartilage specificity gene Sox9 mRNA in the human intervertebral discs

作　　者：赵勇[1] 王文波[1] 卢宇[2] 葛芸[2] 于占革[1] 薛飞[3] 

机构地区：[1]哈尔滨医科大学附属第一医院骨科,150001 [2]哈尔滨市第一医院 [3]中国农业科学院哈尔滨兽医研究所

出　　处：《中华外科杂志》2006年第24期1704-1707,共4页Chinese Journal of Surgery

基　　金：国家自然科学基金资助项目(30471741)

摘　　要：目的探讨白细胞介素1(interleukin-1,IL-1)对人椎间盘细胞软骨特异性基因Sox9和Ⅱ型胶原基因表达的调节作用。方法应用RT-PCR技术检测IL-1对培养的椎间盘细胞中软骨特异性基因Sox9和Ⅱ型胶原基因mRNA表达的调节作用。结果在IL-1浓度为0.1ng/ml、1ng/ml和10ng/ml培养24h时,其对椎间盘细胞Sox9和Ⅱ型胶原基因mRNA可起到显著的负向调控作用;10ng/ml的IL-1随着培养时间的延长对椎间盘细胞中Sox9和Ⅱ型胶原基因mRNA出现显著的负向调控作用。结论IL-1可以按照剂量及时间依赖方式负向调节椎间盘细胞Sox9和Ⅱ型胶原基因的表达。Objective To assess the regulating effects of interleukin-1 （ IL-1 ） on gene expression of cartilage specificity gene Sox9 and type Ⅱ collagen mRNA in the human intervertebral discs. Methods RT-PCR were used to investigate the effects of IL-1 on gene expression of Sox9 and type Ⅱ collagen mRNA in intervertebral discs cells cultures of embryo. Results The Sox9 and type Ⅱ collagen mRNA in intervertebral discs were decreased progressively along with the addition concentrations of IL-1 than the controls. And the mRNA of Sox9 and type Ⅱ collagen also markedly decreased with the time of culture. Conclusions IL-1 could cause dose-dependent and time-dependent inhibition effects on Sox9 and type Ⅱ collagen gene expression in human intervertebral discs.

关 键 词：白细胞介素1 SOX基因 胶原 基因表达 椎间盘 

分 类 号：R681.5[医药卫生—骨科学]