家蝇幼虫分泌物抗菌肽的生化特性初步研究  

Biochemical characteristics of the antibacterial peptides in secretion of Musca domestica larvae

作　　者：国果[1] 吴建伟[1] 付萍[1] 张勇[1] 宋玉竹[1] 宋智魁[1] 

机构地区：[1]贵阳医学院寄生虫学教研室,贵阳550004

出　　处：《昆虫学报》2006年第6期918-923,共6页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(39970087);贵州省教育厅项目(黔教科2003108);贵州省省长专项基金[黔科教办(2003)04];贵州省科学技术基金项目[黔科通(2005)85]

摘　　要：研究了不同温度、蛋白酶及反复冻溶对家蝇Musca domestica幼虫活体浸泡法获得的分泌物抗菌肽抗菌活性的影响;并检测其凝血效应;试管稀释法测定其最低抑菌浓度(MIC)、最低杀菌浓度(MBC);SDS-PAGE分析其分子量范围。结果表明,该抗菌肽具有较强的热稳定性、酶稳定性及较强抗菌活性的特性,无凝血作用。对大肠埃希菌的最低抑菌浓度为37μg/mL、最低杀菌浓度为75μg/mL;分子量约10kD。The influences of temperature, proteinases on antibacterial activity of the antibacterial peptides soaked out from living housefly （ Musca domestica ） larvae were investigated. Mean while, the blood clotting activity, minimal inhibitory concentration （MIC）, minimal bactericidal concentration （MBC） and the molecular weight of these peptides were examined. The results showed that the antibacterial peptides had high heat stability, proteolytic stability and antibacterial activity but without blood clotting activity. The MIC and MBC of these peptides were 37 μg/mL and 75μg/mL, respectively. Their molecular weight was about 10 kD.

关 键 词：家蝇 分泌物 抗菌肽 抗菌活性 生物学特性 

分 类 号：Q965[生物学—昆虫学]